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rdf:type |
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lifeskim:mentions |
umls-concept:C0005767,
umls-concept:C0014644,
umls-concept:C0020792,
umls-concept:C0030956,
umls-concept:C0039194,
umls-concept:C0085358,
umls-concept:C0086418,
umls-concept:C1332714,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1522240,
umls-concept:C1706438,
umls-concept:C2698600
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pubmed:issue |
21
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pubmed:dateCreated |
1999-11-24
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pubmed:abstractText |
The broad clinical implementation of cancer vaccines targeting the induction of specific T cell-mediated immunity is hampered because T cell defined tumor-associated peptides are currently available for only a restricted range of tumor types. Current epitope identification strategies require a priori the generation of T "indicator" cell lines that specifically recognize the tumor antigenic epitope in in vitro assay systems. An alternative to this strategy is the use of "memory" T cells freshly isolated from the peripheral blood of patients with cancer in concert with sensitive effector cell readout assays (such as the cytokine enzyme-linked immunospot assay) and MS to identify relevant peptide epitopes. In a model system, we have evaluated the capacity of natural Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cell line-extracted peptides to activate "memory" viral-specific CD4(+) or CD8(+) T cells freshly isolated from the blood of an EBV-seropositive individual using the IFN-gamma enzyme-linked immunospot assay. After HPLC fractionation and loading onto autologous dendritic cells, multiple naturally processed HLA class I and II-associated lymphoblastoid cell line-derived peptides were isolated that were capable of inducing IFN-gamma spot production by "memory" T lymphocytes. Using MS analysis on a HPLC fraction recognized by CD8(+) T cells, we were able to sequence natural 9-, 10-, and 11-mer peptides naturally processed from the latent EBV antigen LMP-2 (latent membrane protein-2) and presented in the context of HLA-A2. This approach provides a useful methodology for the future identification of MHC-presented viral and tumor epitopes using freshly isolated patient materials.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-1517561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-1719531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-1721637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-1853786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-2113474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-3131436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7506576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7521394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7530012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7531143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7693972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-7890324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-8598464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-8609400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-8892659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-8971014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9120290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9143694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9149807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9151898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9263004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9294140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9368778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9419450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9464798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9521319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9584143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9722921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10518571-9973448
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12033-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10518571-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10518571-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10518571-Cell Line, Transformed,
pubmed-meshheading:10518571-Chromatography, High Pressure Liquid,
pubmed-meshheading:10518571-Herpesvirus 4, Human,
pubmed-meshheading:10518571-Histocompatibility Antigens Class I,
pubmed-meshheading:10518571-Histocompatibility Antigens Class II,
pubmed-meshheading:10518571-Humans,
pubmed-meshheading:10518571-Immunoblotting,
pubmed-meshheading:10518571-Immunologic Memory,
pubmed-meshheading:10518571-Immunologic Techniques,
pubmed-meshheading:10518571-Interferon-gamma,
pubmed-meshheading:10518571-Mass Spectrometry,
pubmed-meshheading:10518571-Peptides,
pubmed-meshheading:10518571-Recombinant Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Identification of naturally processed and HLA-presented Epstein-Barr virus peptides recognized by CD4(+) or CD8(+) T lymphocytes from human blood.
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pubmed:affiliation |
Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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