Source:http://linkedlifedata.com/resource/pubmed/id/10517594
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2000-1-10
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| pubmed:databankReference | |
| pubmed:abstractText |
A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant (fet3delta/fet3delta) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3delta mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3delta mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
145 ( Pt 9)
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2415-22
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10517594-Amino Acid Sequence,
pubmed-meshheading:10517594-Animals,
pubmed-meshheading:10517594-Candida albicans,
pubmed-meshheading:10517594-Candidiasis,
pubmed-meshheading:10517594-Cloning, Molecular,
pubmed-meshheading:10517594-Copper,
pubmed-meshheading:10517594-Gene Deletion,
pubmed-meshheading:10517594-Genes, Fungal,
pubmed-meshheading:10517594-Genetic Complementation Test,
pubmed-meshheading:10517594-Humans,
pubmed-meshheading:10517594-Iron,
pubmed-meshheading:10517594-Mice,
pubmed-meshheading:10517594-Molecular Sequence Data,
pubmed-meshheading:10517594-Oxidoreductases,
pubmed-meshheading:10517594-Plasmids,
pubmed-meshheading:10517594-Polymerase Chain Reaction,
pubmed-meshheading:10517594-Saccharomyces cerevisiae,
pubmed-meshheading:10517594-Sequence Analysis, DNA,
pubmed-meshheading:10517594-Transformation, Genetic
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| pubmed:year |
1999
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| pubmed:articleTitle |
A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption.
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| pubmed:affiliation |
Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Jena, Germany. reck@pmail.hki-jena.de
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| pubmed:publicationType |
Journal Article
|