Source:http://linkedlifedata.com/resource/pubmed/id/10517492
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
1999-10-26
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| pubmed:abstractText |
To achieve long-term expression of human interferon alpha-5 (IFNalpha) gene in the bone marrow (BM) hematopoietic microenvironment, replication-deficient retroviral vector LSN-IFNalpha was used to deliver the IFNalpha gene into human BM CD34+ cells. After fibronectin-facilitated transduction, a fraction of CD34+ cells was plated in methylcellulose medium with or without G418 to assess transduction efficiency and the effect of IFNalpha gene transfer on colony formation. Colony-forming assay in the presence of G418 (400 microg/mL) revealed that 41% CFU-GM colonies are G418 resistant after infection with LSN-IFNalpha retrovirus. There was no significant difference in CFU-GM/BFU-E colony formation among IFNalpha gene-transduced CD34+ cells, control vector (LXSN) transduced-CD34+ cells and nontransduced CD34+ cells. Another portion of CD34+ cells was grown in liquid medium to measure IFNalpha production. RIA revealed that IFNalpha gene-transduced CD34+ cells produced 72.2 +/- 15.4 U/mL (10(6) cells/24 hours) of IFNalpha compared with 8.3 +/- 2.1 U/mL and 4.3 +/- 1.2 U/mL in LXSN-transduced or nontransduced CD34+ cells, respectively. The remaining portion of transduced CD34+ cells was transplanted into immunodeficient (NOD/SCID) mice to allow analysis of long-term expression of IFNalpha. Transplantation of 1x10(6) CD34+ cells into sublethally irradiated NOD/SCID mice showed that IFNalpha and neo(r) mRNA were detectable in engrafted mouse BM cells for up to 6 months. We conclude that continual local expression of IFNalpha in transduced CD34+ cells does not impair either CD34+ cell growth and differentiation or engraftment and long-term survival in NOD/SCID mice.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0301-472X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
27
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1511-8
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:10517492-Animals,
pubmed-meshheading:10517492-Antigens, CD34,
pubmed-meshheading:10517492-Cell Differentiation,
pubmed-meshheading:10517492-Cell Survival,
pubmed-meshheading:10517492-Cells, Cultured,
pubmed-meshheading:10517492-Colony-Forming Units Assay,
pubmed-meshheading:10517492-Female,
pubmed-meshheading:10517492-Gene Expression,
pubmed-meshheading:10517492-Genetic Vectors,
pubmed-meshheading:10517492-Granulocytes,
pubmed-meshheading:10517492-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:10517492-Hematopoietic Stem Cells,
pubmed-meshheading:10517492-Humans,
pubmed-meshheading:10517492-Interferon-alpha,
pubmed-meshheading:10517492-Mice,
pubmed-meshheading:10517492-Mice, Inbred NOD,
pubmed-meshheading:10517492-Mice, SCID,
pubmed-meshheading:10517492-Radioimmunoassay,
pubmed-meshheading:10517492-Retroviridae,
pubmed-meshheading:10517492-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10517492-Transfection
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Human CD34+ hematopoietic cells transduced by retrovirus-mediated interferon alpha gene maintains regeneration capacity and engraftment in NOD/SCID mice.
|
| pubmed:affiliation |
Department of Pharmacology, New York Medical College, Valhalla, NY 10595, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|