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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4 Pt 2
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pubmed:dateCreated |
1999-11-17
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pubmed:abstractText |
The disruption of microtubules by treating bovine aortic endothelial cells with 10(-7)-10(-5) M colchicine caused apoptosis, as evidenced by DNA laddering and TdT-mediated dUTP nick end labeling fluorescence staining. Colchicine treatment also induced a sustained activation of c-Jun NH(2)-terminal kinase (JNK) that lasted for >/=12 h. The blockade of JNK activity by using the negative interfering mutant JNK(K-R) markedly decreased the apoptosis induced by colchicine. Exposure of bovine aortic endothelial cells to laminar shear stress (12 dyn/cm(2)) caused a transient (<2 h) activation of JNK, and there was no induction of apoptosis. The sustained activation of JNK may play a significant role in the apoptosis induced by colchicine.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H1593-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10516199-Animals,
pubmed-meshheading:10516199-Aorta,
pubmed-meshheading:10516199-Apoptosis,
pubmed-meshheading:10516199-Cattle,
pubmed-meshheading:10516199-Cells, Cultured,
pubmed-meshheading:10516199-Colchicine,
pubmed-meshheading:10516199-Endothelium, Vascular,
pubmed-meshheading:10516199-Enzyme Activation,
pubmed-meshheading:10516199-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:10516199-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:10516199-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10516199-Mutation,
pubmed-meshheading:10516199-Stress, Mechanical,
pubmed-meshheading:10516199-ras Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Sustained JNK activation induces endothelial apoptosis: studies with colchicine and shear stress.
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pubmed:affiliation |
Department of Bioengineering, Whitaker Institute of Biomedical Engineering, San Diego, La Jolla, California 92093-0427, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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