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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1999-11-22
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pubmed:abstractText |
Major histocompatibility complex (MHC) molecules play an important role in antigen presentation for induction of tumor as well as cellular and humoral immunities. Recent studies using anti-MHC antibodies demonstrated that antibodies specific for HLA class I molecules induced cellular activation and a type of apoptosis that may be distinct from Fas-dependent or TNFR (tumor necrosis factor-alpha receptor)-dependent processes. We purified a previously untested apoptosis-inducing factor from HL-60 human leukemic cell-conditioned media to homogeneity and sequenced it. It was identified as beta(2)-microglobulin (beta(2)m), which has been previously known as thymotaxin and is a part of the HLA class I antigen complex. beta(2)m acts on both T-leukemic cells and myeloid leukemic cells to induce apoptosis, which then activates caspase 1 and 3. Cross-linking studies showed that biotinilated beta(2)m recognized an epitope distinct from those recognized by the anti-HLA class I antibody, as reported previously. We demonstrated that beta(2)m plays a previously unrecognized and important role in regulating the elimination of tumor cells, which occurs as a result of the action of beta(2)m as an apoptosis-inducing factor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Chloromethyl Ketones,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Fasl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/L 709049,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylvalyl-alanyl-aspart...,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:Douzono-TanakaMM,
pubmed-author:FurukawaYY,
pubmed-author:HatakeKK,
pubmed-author:IkedaKK,
pubmed-author:IkedaMM,
pubmed-author:IshizakiAA,
pubmed-author:KasaharaTT,
pubmed-author:KikuchiJJ,
pubmed-author:KubotaNN,
pubmed-author:LUNTM RMR,
pubmed-author:MannII,
pubmed-author:MishimaYY,
pubmed-author:MoriMM,
pubmed-author:OzawaKK,
pubmed-author:ShimamuraSS,
pubmed-author:TeruiYY,
pubmed-author:TomizukaHH,
pubmed-author:UwaiMM,
pubmed-author:YamadaMM
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2744-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10515878-Amino Acid Chloromethyl Ketones,
pubmed-meshheading:10515878-Amino Acid Sequence,
pubmed-meshheading:10515878-Animals,
pubmed-meshheading:10515878-Antibodies, Monoclonal,
pubmed-meshheading:10515878-Antigens, CD95,
pubmed-meshheading:10515878-Apoptosis,
pubmed-meshheading:10515878-Culture Media, Conditioned,
pubmed-meshheading:10515878-Cysteine Proteinase Inhibitors,
pubmed-meshheading:10515878-Fas Ligand Protein,
pubmed-meshheading:10515878-HL-60 Cells,
pubmed-meshheading:10515878-Humans,
pubmed-meshheading:10515878-K562 Cells,
pubmed-meshheading:10515878-Membrane Glycoproteins,
pubmed-meshheading:10515878-Mice,
pubmed-meshheading:10515878-Molecular Sequence Data,
pubmed-meshheading:10515878-Neoplasm Proteins,
pubmed-meshheading:10515878-Oligopeptides,
pubmed-meshheading:10515878-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:10515878-Sequence Alignment,
pubmed-meshheading:10515878-Sequence Homology, Amino Acid,
pubmed-meshheading:10515878-Tumor Cells, Cultured,
pubmed-meshheading:10515878-Tumor Necrosis Factor-alpha,
pubmed-meshheading:10515878-U937 Cells,
pubmed-meshheading:10515878-beta 2-Microglobulin
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pubmed:year |
1999
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pubmed:articleTitle |
Beta(2)-microglobulin identified as an apoptosis-inducing factor and its characterization.
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pubmed:affiliation |
Department of Hematology and the Division of Molecular Hematopoiesis, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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