Linked Life Data

10514391

Source:http://linkedlifedata.com/resource/pubmed/id/10514391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-11-4
pubmed:abstractText
The mechanisms of pancreatic fibrosis are poorly understood. In the liver, stellate cells play an important role in fibrogenesis. Similar cells have recently been isolated from the pancreas and are termed pancreatic stellate cells. The aim of this study was to determine whether pancreatic stellate cell activation occurs during experimental and human pancreatic fibrosis. Pancreatic fibrosis was induced in rats (n = 24) by infusion of trinitrobenzene sulfonic acid (TNBS) into the pancreatic duct. Surgical specimens were obtained from patients with chronic pancreatitis (n = 6). Pancreatic fibrosis was assessed using the Sirius Red stain and immunohistochemistry for collagen type I. Pancreatic stellate cell activation was assessed by staining for alpha-smooth muscle actin (alphaSMA), desmin, and platelet-derived growth factor receptor type beta (PDGFRbeta). The relationship of fibrosis to stellate cell activation was studied by staining of serial sections for alphaSMA, desmin, PDGFRbeta, and collagen, and by dual-staining for alphaSMA plus either Sirius Red or in situ hybridization for procollagen alpha(1) (I) mRNA. The cellular source of TGFbeta was examined by immunohistochemistry. The histological appearances in the TNBS model resembled those found in human chronic pancreatitis. Areas of pancreatic fibrosis stained positively for Sirius Red and collagen type I. Sirius Red staining was associated with alphaSMA-positive cells. alphaSMA staining colocalized with procollagen alpha(1) (I) mRNA expression. In the rat model, desmin staining was associated with PDGFRbeta in areas of fibrosis. TGFbeta was maximal in acinar cells adjacent to areas of fibrosis and spindle cells within fibrotic bands. Pancreatic stellate cell activation is associated with fibrosis in both human pancreas and in an animal model. These cells appear to play an important role in pancreatic fibrogenesis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-10075961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-2003575, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-2186486, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-2255178, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-2592560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-6204917, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-7657050, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-7768393, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-7851468, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8144642, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8502273, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8549875, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8550043, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8566606, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8682445, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8773917, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-8899803, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9075666, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9126792, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9136846, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9155579, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9207290, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9539653, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9586822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9679048, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9708812, http://linkedlifedata.com/resource/pubmed/commentcorrection/10514391-9758536
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1087-95
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10514391-Actins, pubmed-meshheading:10514391-Animals, pubmed-meshheading:10514391-Chronic Disease, pubmed-meshheading:10514391-Collagen, pubmed-meshheading:10514391-Desmin, pubmed-meshheading:10514391-Disease Models, Animal, pubmed-meshheading:10514391-Fibrosis, pubmed-meshheading:10514391-Glial Fibrillary Acidic Protein, pubmed-meshheading:10514391-Humans, pubmed-meshheading:10514391-Immunohistochemistry, pubmed-meshheading:10514391-Male, pubmed-meshheading:10514391-Pancreas, pubmed-meshheading:10514391-Pancreatitis, Alcoholic, pubmed-meshheading:10514391-Procollagen, pubmed-meshheading:10514391-RNA, Messenger, pubmed-meshheading:10514391-Rats, pubmed-meshheading:10514391-Receptor, Platelet-Derived Growth Factor beta, pubmed-meshheading:10514391-Transforming Growth Factor beta, pubmed-meshheading:10514391-Trinitrobenzenesulfonic Acid
pubmed:year
1999
pubmed:articleTitle
Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis.
pubmed:affiliation
Department of Gastroenterology, Pancreatic Research Group, Prince of Wales Hospital, University of New South Wales, Sydney, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't