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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-1-4
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pubmed:abstractText |
1 Rat foetal distal lung epithelial cells were plated onto permeable supports where they became integrated into epithelial sheets that spontaneously generated short circuit current (ISC). 2 Apical ATP (100 microM) evoked a transient fall in ISC that was followed by a rise to a clear peak which, in turn, was succeeded by a slowly developing decline to a value below control. Apical UTP evoked an essentially identical response. 3 UDP and ADP were ineffective whilst ATP had no effect when added to the basolateral solution. These effects thus appear to be mediated by apical P2Y2 receptors. 4 The rising phase of the responses to ATP/UTP was selectively inhibited by anion transport inhibitors but persisted in the presence of amiloride, which abolished the inhibitory effects of both nucleotides. Thus, apical nucleotides appear to evoke a transient stimulation of anion secretion and sustained inhibition of Na+ absorption. 5 Basolateral isoprenaline (10 microM) elicited a rise in ISC but subsequent addition of apical ATP reversed this effect. Conversely, isoprenaline restored ISC to its basal level following stimulation with ATP. Apical P2Y2 receptors and basolateral beta-adrenoceptors thus allow their respective agonists to exert mutually opposing effects on ISC.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-10066896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-10199813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-1371880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-1872411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-1928320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-205826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-2138778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-2303453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-2663854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-2726315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-3795077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-4443921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-6655575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7508184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7526700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7532370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7541313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7543698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-7811468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8136152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8214015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8381210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8589728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8700127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8764233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8769822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-8928808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9227415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9458813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9585012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9607939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9688838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9688947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9814989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9875854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510438-9886914
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic beta-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/P2ry2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2Y2,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Triphosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
293-300
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10510438-Adenosine Triphosphate,
pubmed-meshheading:10510438-Adrenergic beta-Agonists,
pubmed-meshheading:10510438-Animals,
pubmed-meshheading:10510438-Calcium,
pubmed-meshheading:10510438-Cells, Cultured,
pubmed-meshheading:10510438-Enzyme Inhibitors,
pubmed-meshheading:10510438-Epithelial Cells,
pubmed-meshheading:10510438-Ion Channels,
pubmed-meshheading:10510438-Isoproterenol,
pubmed-meshheading:10510438-Lung,
pubmed-meshheading:10510438-Rats,
pubmed-meshheading:10510438-Receptors, Purinergic P2,
pubmed-meshheading:10510438-Receptors, Purinergic P2Y2,
pubmed-meshheading:10510438-Thapsigargin,
pubmed-meshheading:10510438-Uridine Triphosphate
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pubmed:year |
1999
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pubmed:articleTitle |
P2Y2 receptor-mediated inhibition of ion transport in distal lung epithelial cells.
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pubmed:affiliation |
Lung Membrane Transport Group, Tayside Institute of Child Health, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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