Linked Life Data

10510371

Source:http://linkedlifedata.com/resource/pubmed/id/10510371

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1999-11-4
pubmed:abstractText
Whether memory B cells possess altered differentiative potentials and respond in a qualitatively distinct fashion to extrinsic signals as compared with their naive precursors is a current subject of debate. We have investigated this issue by examining the participation of a predominant anti-arsonate clonotype in the primary and secondary responses in the spleens of A/J mice. While this clonotype gives rise to few Ab-forming cells (AFC) in the primary response, shortly after secondary immunization its memory cell progeny produce a massive splenic IgG AFC response, largely in the red pulp. Extensive clonal expansion and migration take place during the secondary AFC response but Ab V region somatic hypermutation is not reinduced. The primary and secondary germinal center (GC) responses of this clonotype are both characterized by ongoing V gene hypermutation and phenotypic selection, little or no inter-GC migration, and derivation of multiple, spatially distinct GCs from a single progenitor. However, the kinetics of these responses differ, with V genes containing a high frequency of total as well as affinity-enhancing mutations appearing rapidly in secondary GCs, suggesting either recruitment of memory cells into this response, or accelerated rates of hypermutation and selection. In contrast, the frequency of mutation observed per V gene does not increase monotonically during the primary GC response of this clonotype, suggesting ongoing emigration of B cells that have sustained affinity- and specificity-enhancing mutations.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4315-27
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10510371-Animals, pubmed-meshheading:10510371-Antibody-Producing Cells, pubmed-meshheading:10510371-Arsenicals, pubmed-meshheading:10510371-B-Lymphocytes, pubmed-meshheading:10510371-Base Sequence, pubmed-meshheading:10510371-Cell Division, pubmed-meshheading:10510371-Cell Movement, pubmed-meshheading:10510371-Clone Cells, pubmed-meshheading:10510371-Genes, Immunoglobulin, pubmed-meshheading:10510371-Germinal Center, pubmed-meshheading:10510371-Haptens, pubmed-meshheading:10510371-Hemocyanin, pubmed-meshheading:10510371-Immunization, pubmed-meshheading:10510371-Immunization, Secondary, pubmed-meshheading:10510371-Immunoglobulin Heavy Chains, pubmed-meshheading:10510371-Immunoglobulin Variable Region, pubmed-meshheading:10510371-Immunologic Memory, pubmed-meshheading:10510371-Immunophenotyping, pubmed-meshheading:10510371-Mice, pubmed-meshheading:10510371-Mice, Inbred A, pubmed-meshheading:10510371-Molecular Sequence Data, pubmed-meshheading:10510371-Mollusca, pubmed-meshheading:10510371-Mutation, pubmed-meshheading:10510371-Signal Transduction
pubmed:year
1999
pubmed:articleTitle
Contrasting the in situ behavior of a memory B cell clone during primary and secondary immune responses.
pubmed:affiliation
Kimmel Cancer Institute, Department of Microbiology, Jefferson Medical College, Philadelphia, PA 19107, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.