Linked Life Data

10509354

Source:http://linkedlifedata.com/resource/pubmed/id/10509354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-9-10
pubmed:abstractText
The purpose of the present study was to determine the distribution of granulocyte-macrophage colony-stimulating factor (GM-CSF) and its receptor (GM-CSF-R) in the human fetus. We used reverse transcription PCR to detect GM-CSF and GM-CSF-R mRNA in human fetal organs at 8 and 16 wk postconception, and cell-specific protein expression was localized in tissues by immunohistochemistry. GM-CSF was also measured by ELISA in paired samples of spinal fluid and plasma. GM-CSF mRNA and/or protein were detected in lung macrophages, spleen, adrenal cortex, placenta, and CNS including neurons and astrocytes. GM-CSF was detected by ELISA in 10 of the 39 cerebrospinal fluid samples tested. GM-CSF-R mRNA expression was present in all organs tested. Immunoreactivity for GM-CSF-R in most organs was limited to macrophages, but, brain, neurons and glial cells showed immunoreactivity. We conclude that GM-CSF is produced in lung, spleen, adrenal, placenta, and neural tissues during human fetal development and that GM-CSF-responsive cells include macrophages, neurons, and glial cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0031-3998
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
358-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10509354-Adolescent, pubmed-meshheading:10509354-Base Sequence, pubmed-meshheading:10509354-Child, pubmed-meshheading:10509354-Child, Preschool, pubmed-meshheading:10509354-DNA, Complementary, pubmed-meshheading:10509354-Embryonic and Fetal Development, pubmed-meshheading:10509354-Female, pubmed-meshheading:10509354-Fetus, pubmed-meshheading:10509354-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:10509354-Humans, pubmed-meshheading:10509354-Immunohistochemistry, pubmed-meshheading:10509354-Infant, pubmed-meshheading:10509354-Infant, Newborn, pubmed-meshheading:10509354-Pregnancy, pubmed-meshheading:10509354-RNA, Messenger, pubmed-meshheading:10509354-Receptors, Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:10509354-Tissue Distribution
pubmed:year
1999
pubmed:articleTitle
The distribution of granulocyte-macrophage colony-stimulating factor and its receptor in the developing human fetus.
pubmed:affiliation
Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't