| pubmed-article:10508478 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0302592 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C1622968 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:10508478 | lifeskim:mentions | umls-concept:C0205231 | lld:lifeskim |
| pubmed-article:10508478 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10508478 | pubmed:dateCreated | 1999-11-18 | lld:pubmed |
| pubmed-article:10508478 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:abstractText | Human-papillomavirus (HPV)-E2 protein is involved in gene-expression regulation and replication of HPV genome. Disruption of the E2 gene during viral integration has been proposed as a mechanism of tumoral progression, since the expression of E6/E7 viral oncogenes is allowed. However, retention of E1/E2 genes and high viral amplification are frequently found in HPV16-positive carcinomas of some populations. In this study, we investigated whether retention of E1/E2 and viral amplification are associated with particular HPV16 E2 variants in cervical carcinomas. HPV16 detection, E1/E2 integrity and viral amplification were explored by Southern blot in 123 cervical carcinomas. HPV16 variants were identified by Southern blot and by sequencing E6, L1/MY and E2 regions. Of 46 HPV16-positive tumors, 34 were positive for E1/E2 and 14 of them showed a variant restriction pattern by mutations in E2. All 14 were Asian-American (AA) variants and, of 11 sub-classified, 6 were AA-a and 5 AA-c. Two E1/E2-negative tumors also contained the AA-c variant, while the remaining HPV16-positive tumors contained only European variants. The E2 gene of AA variants showed 24 mutations, 19 identical in both sub-classes. The 24 mutations were distributed throughout the entire gene and 19 result in 18 amino-acid changes. The AA variants were associated with E1/E2-positive carcinomas with more than 50 viral copies/cell (p = 0.035). The association of Asian-American E2 variants with retention of E1/E2 suggests that E2 variation may be an alternative mechanism de-regulating the expression of viral oncogenes. | lld:pubmed |
| pubmed-article:10508478 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10508478 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10508478 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10508478 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:10508478 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:LopezNN | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:GuidoMM | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:CasasLL | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:GalvanS CSC | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:BerumenJJ | lld:pubmed |
| pubmed-article:10508478 | pubmed:author | pubmed-author:OrdoñezR MRM | lld:pubmed |
| pubmed-article:10508478 | pubmed:copyrightInfo | Copyright 1999 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10508478 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10508478 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:10508478 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:10508478 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10508478 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10508478 | pubmed:pagination | 449-55 | lld:pubmed |
| pubmed-article:10508478 | pubmed:dateRevised | 2007-7-24 | lld:pubmed |
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| pubmed-article:10508478 | pubmed:meshHeading | pubmed-meshheading:10508478... | lld:pubmed |
| pubmed-article:10508478 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10508478 | pubmed:articleTitle | Asian-american variants of human papillomavirus type 16 have extensive mutations in the E2 gene and are highly amplified in cervical carcinomas. | lld:pubmed |
| pubmed-article:10508478 | pubmed:affiliation | Multidisciplinary Research Laboratory, Military School of Medical Graduates and Army School of Medicine, University of the Army and Air Force, Mexico City, Mexico. | lld:pubmed |
| pubmed-article:10508478 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10508478 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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