Linked Life Data

10508478

Source:http://linkedlifedata.com/resource/pubmed/id/10508478

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-11-18
pubmed:databankReference
pubmed:abstractText
Human-papillomavirus (HPV)-E2 protein is involved in gene-expression regulation and replication of HPV genome. Disruption of the E2 gene during viral integration has been proposed as a mechanism of tumoral progression, since the expression of E6/E7 viral oncogenes is allowed. However, retention of E1/E2 genes and high viral amplification are frequently found in HPV16-positive carcinomas of some populations. In this study, we investigated whether retention of E1/E2 and viral amplification are associated with particular HPV16 E2 variants in cervical carcinomas. HPV16 detection, E1/E2 integrity and viral amplification were explored by Southern blot in 123 cervical carcinomas. HPV16 variants were identified by Southern blot and by sequencing E6, L1/MY and E2 regions. Of 46 HPV16-positive tumors, 34 were positive for E1/E2 and 14 of them showed a variant restriction pattern by mutations in E2. All 14 were Asian-American (AA) variants and, of 11 sub-classified, 6 were AA-a and 5 AA-c. Two E1/E2-negative tumors also contained the AA-c variant, while the remaining HPV16-positive tumors contained only European variants. The E2 gene of AA variants showed 24 mutations, 19 identical in both sub-classes. The 24 mutations were distributed throughout the entire gene and 19 result in 18 amino-acid changes. The AA variants were associated with E1/E2-positive carcinomas with more than 50 viral copies/cell (p = 0.035). The association of Asian-American E2 variants with retention of E1/E2 suggests that E2 variation may be an alternative mechanism de-regulating the expression of viral oncogenes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0020-7136
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
449-55
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed-meshheading:10508478-Amino Acid Substitution, pubmed-meshheading:10508478-Asian Americans, pubmed-meshheading:10508478-Blotting, Southern, pubmed-meshheading:10508478-Capsid Proteins, pubmed-meshheading:10508478-DNA, Viral, pubmed-meshheading:10508478-DNA Mutational Analysis, pubmed-meshheading:10508478-DNA-Binding Proteins, pubmed-meshheading:10508478-Female, pubmed-meshheading:10508478-Gene Dosage, pubmed-meshheading:10508478-Humans, pubmed-meshheading:10508478-Oncogene Proteins, Viral, pubmed-meshheading:10508478-Papillomaviridae, pubmed-meshheading:10508478-Papillomavirus Infections, pubmed-meshheading:10508478-Point Mutation, pubmed-meshheading:10508478-Repressor Proteins, pubmed-meshheading:10508478-Tumor Virus Infections, pubmed-meshheading:10508478-Uterine Cervical Neoplasms
pubmed:year
1999
pubmed:articleTitle
Asian-american variants of human papillomavirus type 16 have extensive mutations in the E2 gene and are highly amplified in cervical carcinomas.
pubmed:affiliation
Multidisciplinary Research Laboratory, Military School of Medical Graduates and Army School of Medicine, University of the Army and Air Force, Mexico City, Mexico.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't