Linked Life Data

10505057

Source:http://linkedlifedata.com/resource/pubmed/id/10505057

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-10-12
pubmed:abstractText
Oviductal endosalpingeal cells were isolated mechanically from heifers and cultured until there was 100% confluency. The cells were loaded with the Ca(2+)-sensitive fluorochrome, fura-2/acetoxymethylester, and cytosolic free calcium ([Ca2+]i) was monitored by spectrofluorimetry. Platelet-activating factor, at a concentration of 30 nmol l-1, induced an intracellular Ca2+ increase in cultured bovine oviductal cells, mainly via influx from the extracellular space. In fura-2-loaded oviductal cells, different Ca2+ channel blockers were investigated to characterize the pathways responsible for the Ca2+ influx. The negative effects of Ni(2+)-, La(3+)-activated K+ channel blockers, such as apamin and charybdotoxin, and Ca2+ channel blockers, such as dotarizine, on the platelet-activating factor-induced [Ca2+]i increase indicate the minor participation of the voltage-gated Ca2+ channels. TMB-8 and flufenamic acid blocked the platelet-activating factor-induced Ca2+ increase directly on non-selective cationic channels or acted via a Ca2+ release-triggered Ca2+ influx. Platelet-activating factor, at concentrations of 1.25 mumol l-1 and 2.5 mumol l-1, significantly stimulated the proliferation and depolarization of oviductal cells, but 10 mumol l-1 significantly decreased both parameters and exerted a cytotoxic effect on cells. After incubation with TMB-8 or flufenamic acid, the cell proliferation was inhibited in a concentration-dependent manner, with IC50 values of 26.57 mumol l-1 and 95.29 mumol l-1, respectively. The depolarization was significantly inhibited at 50 mumol l-1 for both TMB-8 and flufenamic acid. The results of the present study may contribute to further understanding of the mechanism behind the actions of platelet-activating factor on oviductal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-72
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10505057-Analysis of Variance, pubmed-meshheading:10505057-Animals, pubmed-meshheading:10505057-Calcium, pubmed-meshheading:10505057-Calcium Channel Blockers, pubmed-meshheading:10505057-Calcium Channels, pubmed-meshheading:10505057-Cattle, pubmed-meshheading:10505057-Cell Division, pubmed-meshheading:10505057-Cells, Cultured, pubmed-meshheading:10505057-Dose-Response Relationship, Drug, pubmed-meshheading:10505057-Fallopian Tubes, pubmed-meshheading:10505057-Female, pubmed-meshheading:10505057-Flufenamic Acid, pubmed-meshheading:10505057-Fura-2, pubmed-meshheading:10505057-Gallic Acid, pubmed-meshheading:10505057-Homeostasis, pubmed-meshheading:10505057-Membrane Potentials, pubmed-meshheading:10505057-Microscopy, Fluorescence, pubmed-meshheading:10505057-Microscopy, Phase-Contrast, pubmed-meshheading:10505057-Platelet Activating Factor
pubmed:year
1999
pubmed:articleTitle
Influence of inhibitors on increase in intracellular free calcium and proliferation induced by platelet-activating factor in bovine oviductal cells.
pubmed:affiliation
Unit of Reproductive Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
pubmed:publicationType
Journal Article