Source:http://linkedlifedata.com/resource/pubmed/id/10503783
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
1999-11-2
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| pubmed:abstractText |
The natural occurrence of complement-activating anti-Galalpha1-3Gal antibodies (anti-Gal) in human serum is considered as a major obstacle to xenotransplantation. In this study we determined anti-Gal in sera of 200 healthy volunteers (100 male, 100 female) of different age groups using an IgG- and IgM-isotype specific ELISA. In addition, we used a direct hemagglutination test involving rabbit erythrocytes (E(R)), which are known for abundant surface expression of the Galalpha1-3Gal antigen. The measured arbitrary ELISA units (U) for anti-Gal ranged from 5 to 204 U (77+/-47; mean +/- 1 SD) for IgM, and from 1 to 162 U(73+/-32) for IgG. Anti-E(R) hemagglutination titers were between 1: 65536 and 1: 64 (mean 1: 703), with 75% of all serum samples being in the range of 1: 8192-1: 512. Specificity of the tests was determined by immunoabsorption of anti-Gal on Sepharose-coupled synthetic Galalpha1-3Gal antigen, which reduced ELISA as well as agglutination titers by 90% or more. Overall, inter-individual differences of both anti-Gal ELISA values and E(R) agglutination titers exceeded age-, gender-, or ABO-type related changes. A tendency was found to higher anti-Gal IgM values in women than in men (P < 0.005 by Student's t-test), whereas the gender-difference for anti-Gal IgG or E(R) titers was not significant. Application of the anti-Gal ELISA as well as the E(R) agglutination assay for pre-transplant screening of potential pig xenograft recipients is discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Heterophile,
http://linkedlifedata.com/resource/pubmed/chemical/Disaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/galactosyl-(1-3)galactose
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0908-665X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
173-80
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10503783-Adult,
pubmed-meshheading:10503783-Aged,
pubmed-meshheading:10503783-Animals,
pubmed-meshheading:10503783-Antibodies, Heterophile,
pubmed-meshheading:10503783-Disaccharides,
pubmed-meshheading:10503783-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10503783-Epitopes,
pubmed-meshheading:10503783-Female,
pubmed-meshheading:10503783-Graft Rejection,
pubmed-meshheading:10503783-Humans,
pubmed-meshheading:10503783-Immunoglobulin G,
pubmed-meshheading:10503783-Immunoglobulin M,
pubmed-meshheading:10503783-Male,
pubmed-meshheading:10503783-Middle Aged,
pubmed-meshheading:10503783-Organ Transplantation,
pubmed-meshheading:10503783-Rabbits,
pubmed-meshheading:10503783-Transplantation, Heterologous,
pubmed-meshheading:10503783-Transplantation Immunology
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| pubmed:year |
1999
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| pubmed:articleTitle |
Quantitation and characterization of anti-Galalpha1-3Gal antibodies in sera of 200 healthy persons.
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| pubmed:affiliation |
Department of Hematology and Regional Red Cross Blood Transfusion Center, Bern, Switzerland.
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| pubmed:publicationType |
Journal Article
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