pubmed-article:10500252 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C0008633 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C1708726 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C1412467 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C1422410 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C1516050 | lld:lifeskim |
pubmed-article:10500252 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
pubmed-article:10500252 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:10500252 | pubmed:dateCreated | 1999-11-5 | lld:pubmed |
pubmed-article:10500252 | pubmed:abstractText | The gene (HSD-1) coding a human sperm membrane protein (hSMP-1) was isolated from a human testis cDNA expression library using antibodies found in the serum of an infertile woman. HSD-1 was localized to a single locus on chromosome 9 and assigned to band 9p12-p13 by fluorescent in situ hybridization (FISH) mapping and DAPI (4,6-diamidino-2-phenylindole) banding, using rat/human somatic cell hybrids and metaphase chromosomes of human lymphocytes. In rescreening a testis lambdagt10 cDNA expression library, the full-length cDNA (HSD-1) and several truncated cDNAs with heterologous regions were isolated from positive clones. The heterology consisted of deletion, insertion and alteration of the 5'-end. These heterologous truncated fragments may be produced by alternative splicing of mRNAs. Two recombinant prokaryotic expression vectors were constructed with one of the heterologous fragment (clone #26) with and without the alternative 5'-end. Escherichia coli transfected with the construct containing the alternative 5'-end failed to produce the recombinant product, whereas those transfected with the vector lacking the 5'-end produced hSMP-1. DNASIS analysis of the structure of #26 mRNA suggests that the 5'-end has a stable secondary configuration that may maintain the mRNA in an inactivated state, whereby hindering its translation and preventing the expression of the gene. | lld:pubmed |
pubmed-article:10500252 | pubmed:language | eng | lld:pubmed |
pubmed-article:10500252 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10500252 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:10500252 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:10500252 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:10500252 | pubmed:month | Oct | lld:pubmed |
pubmed-article:10500252 | pubmed:issn | 0006-3002 | lld:pubmed |
pubmed-article:10500252 | pubmed:author | pubmed-author:KoideS SSS | lld:pubmed |
pubmed-article:10500252 | pubmed:author | pubmed-author:WangLL | lld:pubmed |
pubmed-article:10500252 | pubmed:author | pubmed-author:WangHH | lld:pubmed |
pubmed-article:10500252 | pubmed:author | pubmed-author:ChenDD | lld:pubmed |
pubmed-article:10500252 | pubmed:author | pubmed-author:MiaoSS | lld:pubmed |
pubmed-article:10500252 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:10500252 | pubmed:day | 6 | lld:pubmed |
pubmed-article:10500252 | pubmed:volume | 1447 | lld:pubmed |
pubmed-article:10500252 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:10500252 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:10500252 | pubmed:pagination | 119-24 | lld:pubmed |
pubmed-article:10500252 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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pubmed-article:10500252 | pubmed:year | 1999 | lld:pubmed |
pubmed-article:10500252 | pubmed:articleTitle | Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a human sperm membrane protein (hSMP-1). | lld:pubmed |
pubmed-article:10500252 | pubmed:affiliation | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Faculty of Basic Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR China. | lld:pubmed |
pubmed-article:10500252 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:10500252 | pubmed:publicationType | Comparative Study | lld:pubmed |
pubmed-article:10500252 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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