Source:http://linkedlifedata.com/resource/pubmed/id/10500252
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1999-11-5
|
| pubmed:abstractText |
The gene (HSD-1) coding a human sperm membrane protein (hSMP-1) was isolated from a human testis cDNA expression library using antibodies found in the serum of an infertile woman. HSD-1 was localized to a single locus on chromosome 9 and assigned to band 9p12-p13 by fluorescent in situ hybridization (FISH) mapping and DAPI (4,6-diamidino-2-phenylindole) banding, using rat/human somatic cell hybrids and metaphase chromosomes of human lymphocytes. In rescreening a testis lambdagt10 cDNA expression library, the full-length cDNA (HSD-1) and several truncated cDNAs with heterologous regions were isolated from positive clones. The heterology consisted of deletion, insertion and alteration of the 5'-end. These heterologous truncated fragments may be produced by alternative splicing of mRNAs. Two recombinant prokaryotic expression vectors were constructed with one of the heterologous fragment (clone #26) with and without the alternative 5'-end. Escherichia coli transfected with the construct containing the alternative 5'-end failed to produce the recombinant product, whereas those transfected with the vector lacking the 5'-end produced hSMP-1. DNASIS analysis of the structure of #26 mRNA suggests that the 5'-end has a stable secondary configuration that may maintain the mRNA in an inactivated state, whereby hindering its translation and preventing the expression of the gene.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SPAG8 protein, human
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0006-3002
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
6
|
| pubmed:volume |
1447
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
119-24
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10500252-Amino Acid Sequence,
pubmed-meshheading:10500252-Antigens, Surface,
pubmed-meshheading:10500252-Base Sequence,
pubmed-meshheading:10500252-Chromosomes, Human, Pair 9,
pubmed-meshheading:10500252-Consensus Sequence,
pubmed-meshheading:10500252-DNA, Complementary,
pubmed-meshheading:10500252-Escherichia coli,
pubmed-meshheading:10500252-Gene Library,
pubmed-meshheading:10500252-Humans,
pubmed-meshheading:10500252-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10500252-Lymphocytes,
pubmed-meshheading:10500252-Male,
pubmed-meshheading:10500252-Membrane Proteins,
pubmed-meshheading:10500252-Molecular Sequence Data,
pubmed-meshheading:10500252-Nucleic Acid Conformation,
pubmed-meshheading:10500252-RNA, Messenger,
pubmed-meshheading:10500252-Testis,
pubmed-meshheading:10500252-Transfection
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Assignment of chromosomal locus and evidence for alternatively spliced mRNAs of a human sperm membrane protein (hSMP-1).
|
| pubmed:affiliation |
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Faculty of Basic Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR China.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|