Linked Life Data

10498603

Source:http://linkedlifedata.com/resource/pubmed/id/10498603

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1999-11-4
pubmed:abstractText
PU.1 is a hematopoietic cell-specific ets family transcription factor. Gene disruption of PU.1 results in a cell autonomous defect in hematopoietic progenitor cells that manifests as abnormal myeloid and B-lymphoid development. Of the myeloid lineages, no mature macrophages develop, and the neutrophils that develop are aberrantly and incompletely matured. One of the documented abnormalities of PU. 1 null (deficient) hematopoietic cells is a failure to express receptors for granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM)-CSF, and M-CSF. To elucidate the roles of the myeloid growth factor receptors in myeloid cell differentiation, and to distinguish their role from that of PU.1, we have restored expression of the G- and M-CSF receptors in PU.1-deficient cells using retroviral vectors. We have similarly expressed PU.1 in these cells. Whereas expression of growth factor receptors merely allows a PU.1-deficient cell line to survive and grow in the relevant growth factor, expression of PU.1 enables the development of F4/80(+), Mac-1(+)/CD11b(+) macrophages, expression of gp91(phox) and generation of superoxide, and expression of secondary granule genes for neutrophil collagenase and gelatinase. These studies reinforce the idea that availability of PU.1 is crucial for normal myeloid development and clarify some of the molecular events in developing neutrophils and macrophages that are critically dependent on PU.1.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2310-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10498603-Animals, pubmed-meshheading:10498603-Cell Differentiation, pubmed-meshheading:10498603-Cell Line, pubmed-meshheading:10498603-Genetic Vectors, pubmed-meshheading:10498603-Hematopoietic Stem Cells, pubmed-meshheading:10498603-Liver, pubmed-meshheading:10498603-Macrophages, pubmed-meshheading:10498603-Mice, pubmed-meshheading:10498603-Mice, Knockout, pubmed-meshheading:10498603-Proto-Oncogene Proteins, pubmed-meshheading:10498603-Receptor, Macrophage Colony-Stimulating Factor, pubmed-meshheading:10498603-Receptors, Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:10498603-Recombinant Proteins, pubmed-meshheading:10498603-Retroviridae, pubmed-meshheading:10498603-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10498603-Trans-Activators, pubmed-meshheading:10498603-Transfection
pubmed:year
1999
pubmed:articleTitle
PU.1 and the granulocyte- and macrophage colony-stimulating factor receptors play distinct roles in late-stage myeloid cell differentiation.
pubmed:affiliation
The Burnham Institute, La Jolla, CA, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't