Linked Life Data

10494963

Source:http://linkedlifedata.com/resource/pubmed/id/10494963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-12-3
pubmed:abstractText
Influenza vaccine production is dependent on the availability of embryonated hen eggs for virus growth. This is an extremely cumbersome system with many disadvantages with respect to selection of virus variants and the presence of adventitious viruses. We have developed an alternative cell culture system which allows rapid production of large volumes of vaccine. The WHO-approved Vero cell line was used in serum-free culture to grow many influenza strains to high titre. This system could be scaled-up to allow vaccine production with a 1200 litre fermenter. A purification scheme was developed which resulted in a high purity whole virus vaccine. This was demonstrated to be at least as immunogenic as a conventional egg-derived preparation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-10; discussion 111
pubmed:dateRevised
2005-11-22
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Development of a Vero cell-derived influenza whole virus vaccine.
pubmed:affiliation
Biomedical Research Center, Immuno AG, Orth/Donau, Austria.
pubmed:publicationType
Journal Article