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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-10-21
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pubmed:abstractText |
We have studied two anti-p185 antibodies: the monoclonal antibody 7. 16.4 and rhuMAb 4D5, which were raised against the the ectodomain of rat (p185(neu)), and the human (p185(her2/neu)) homolog, respectively. Studies on the structure of these two antibodies indicate that they share structural similarity in the variable region, especially the CDR3 region, which determines the antibody-antigen interaction. Further studies by flow cytometry revealed that 7.16.4 can compete with rhuMAb4D5 for binding to the cell surface p185(her2/neu), suggesting that these two antibodies share an epitope on the p185 receptor. Furthermore, 7.16.4 can also inhibit proliferation and transformation caused by p185(her2/neu). Moreover the rhuMAb 4D5 binds to the rat p185(neu). With the observation that 7.16.4 positively stains human breast cancer tissues that overexpress p185(her2/neu), 7.16.4 may be useful for the pathological diagnosis and therapy of human tumors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, Humanized,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue,
http://linkedlifedata.com/resource/pubmed/chemical/trastuzumab
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-4800
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-25
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10493889-3T3 Cells,
pubmed-meshheading:10493889-Animals,
pubmed-meshheading:10493889-Antibodies, Monoclonal,
pubmed-meshheading:10493889-Antibodies, Monoclonal, Humanized,
pubmed-meshheading:10493889-Binding Sites, Antibody,
pubmed-meshheading:10493889-Breast Neoplasms,
pubmed-meshheading:10493889-Cell Transformation, Neoplastic,
pubmed-meshheading:10493889-Cross Reactions,
pubmed-meshheading:10493889-Epitopes,
pubmed-meshheading:10493889-Female,
pubmed-meshheading:10493889-Flow Cytometry,
pubmed-meshheading:10493889-Humans,
pubmed-meshheading:10493889-Immunoenzyme Techniques,
pubmed-meshheading:10493889-Immunoglobulin Variable Region,
pubmed-meshheading:10493889-Male,
pubmed-meshheading:10493889-Mice,
pubmed-meshheading:10493889-Models, Molecular,
pubmed-meshheading:10493889-Prostatic Neoplasms,
pubmed-meshheading:10493889-Rats,
pubmed-meshheading:10493889-Receptor, erbB-2,
pubmed-meshheading:10493889-Tetrazolium Salts,
pubmed-meshheading:10493889-Thiazoles
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pubmed:year |
1999
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pubmed:articleTitle |
Shared antigenic epitopes and pathobiological functions of anti-p185(her2/neu) monoclonal antibodies.
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pubmed:affiliation |
Department of Pathology, School of Medicine, University of Pennsylvania, 36th Hamilton Walk, Philadelphia, Pennsylvania 19104, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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