| pubmed-article:10493819 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10493819 | lifeskim:mentions | umls-concept:C0034823 | lld:lifeskim |
| pubmed-article:10493819 | lifeskim:mentions | umls-concept:C0031669 | lld:lifeskim |
| pubmed-article:10493819 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:10493819 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:10493819 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:10493819 | pubmed:issue | 38 | lld:pubmed |
| pubmed-article:10493819 | pubmed:dateCreated | 1999-10-28 | lld:pubmed |
| pubmed-article:10493819 | pubmed:abstractText | Binding of lutropin/choriogonadotropin (LH/CG) to its cognate receptor results in the activation of adenylyl cyclase and phospholipase C. This divergent signaling of the LH receptor is based on the independent activation of distinct G protein subfamilies, i.e. , Gs, Gi, and potentially also Gq. To examine the selectivity of LH receptor coupling to phospholipase C beta-activating G proteins, we used an in vivo reconstitution system based on the coexpression of the LH receptor and different G proteins in baculovirus-infected insect cells. In this paper, we describe a refined expression strategy for the LH receptor in insect cells. The receptor protein was inserted into the cell membrane at an expression level of 0.8 pmol/mg of membrane protein. Sf9 cells expressing the LH receptor responded to hCG challenge with a concentration-dependent accumulation of intracellular cAMP (EC50 = 630 nM) but not of inositol phosphates, whereas stimulation of the histamine H1 receptor in Sf9 cells led to increased phospholipase C (PLC) activity. Immunoblotting experiments using G protein-specific antisera revealed the absence of quantitative amounts of alpha i in Sf9 cells, whereas alpha s and alpha q/11 were detected. We therefore attempted to restore the hCG-dependent PLC activation by infection of Sf9 cells with viruses encoding the LH receptor and different G protein alpha subunits. HCG stimulation of cells coexpressing the LH receptor and exogenous alpha i2 resulted in stimulation of PLC activity. In cells coinfected with an alpha i3-baculovirus, hCG challenge led to a minor activation of PLC, whereas no hCG-dependent PLC stimulation was observed in cells coexpressing alpha i1. Most notably, coinfection with baculoviruses encoding alpha q or alpha 11 did not reproduce the PLC activation by the LH receptor. Thus, the murine LH receptor activates adenylyl cyclase via Gs and PLC via selective coupling to Gi2. | lld:pubmed |
| pubmed-article:10493819 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10493819 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10493819 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10493819 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10493819 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:10493819 | pubmed:author | pubmed-author:GudermannTT | lld:pubmed |
| pubmed-article:10493819 | pubmed:author | pubmed-author:KühnBB | lld:pubmed |
| pubmed-article:10493819 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10493819 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:10493819 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:10493819 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10493819 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10493819 | pubmed:pagination | 12490-8 | lld:pubmed |
| pubmed-article:10493819 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10493819 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10493819 | pubmed:articleTitle | The luteinizing hormone receptor activates phospholipase C via preferential coupling to Gi2. | lld:pubmed |
| pubmed-article:10493819 | pubmed:affiliation | Institut für Pharmakologie, Freie Universität Berlin, Germany. | lld:pubmed |
| pubmed-article:10493819 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10493819 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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