Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
19
pubmed:dateCreated
1999-10-14
pubmed:abstractText
Oligodendrocytes (OLs) extend processes to contact axons as a prerequisite step in myelin formation. As the OL processes migrate toward their axonal targets, they modify adhesion to their substrate, an event that may be regulated by matrix metalloproteinases (MMPs). In the mouse optic nerve, MMP-9/gelatinase B increases during myelin formation. Although tissue inhibitor of metalloproteinase (TIMP)-3 also increases in parallel, the developing optic nerve has focally active MMPs demonstrable by in situ zymography. The distribution of proteolytic activity is similar to that of myelin basic protein, a marker of myelin formation. OLs in culture secrete MMP-9 and express active cell-associated metalloproteinases at the growing tips of their processes. TIMP-1 and a function-perturbing anti-MMP-9 antibody attenuate outgrowth of processes by OLs, indicating a requirement for MMP-9 in process outgrowth. Process reformation is retarded significantly in OLs cultured from MMP-9 null mice, as compared with controls, providing genetic evidence that MMP-9 is necessary for process outgrowth. These data show that MMP-9 facilitates process outgrowth by OLs in vivo and in culture.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8464-75
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10493747-Adult, pubmed-meshheading:10493747-Aging, pubmed-meshheading:10493747-Animals, pubmed-meshheading:10493747-Axons, pubmed-meshheading:10493747-Brain, pubmed-meshheading:10493747-Cells, Cultured, pubmed-meshheading:10493747-Collagenases, pubmed-meshheading:10493747-Gelatinases, pubmed-meshheading:10493747-Gene Expression Regulation, Developmental, pubmed-meshheading:10493747-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10493747-Humans, pubmed-meshheading:10493747-Matrix Metalloproteinase 2, pubmed-meshheading:10493747-Matrix Metalloproteinase 9, pubmed-meshheading:10493747-Metalloendopeptidases, pubmed-meshheading:10493747-Mice, pubmed-meshheading:10493747-Mice, Knockout, pubmed-meshheading:10493747-Myelin Sheath, pubmed-meshheading:10493747-Oligodendroglia, pubmed-meshheading:10493747-Optic Nerve
pubmed:year
1999
pubmed:articleTitle
Matrix metalloproteinase-9/gelatinase B is required for process outgrowth by oligodendrocytes.
pubmed:affiliation
Departments of Oncology and Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't