Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-10-12
pubmed:abstractText
In Saccharomyces cerevisiae, amino acid permeases are divided into two classes. One class, represented by the general amino acid permease GAP1, contains permeases regulated in response to the nitrogen source. The other class, including the high affinity tryptophan permease, TAT2, consists of the so-called constitutive permeases. We show that TAT2 is regulated at the level of protein stability. In exponentially growing cells, TAT2 is in the plasma membrane and also accumulates in internal compartments of the secretory pathway. Upon nutrient deprivation or rapamycin treatment, TAT2 is transported to and degraded in the vacuole. The ubiquitination machinery and lysine residues within the NH(2)-terminal 31 amino acids of TAT2 mediate ubiquitination and degradation of the permease. Starvation-induced degradation of internal TAT2 is blocked in sec18, sec23, pep12, and vps27 mutants, but not in sec4, end4, and apg1 mutants, suggesting that, upon nutrient limitation, internal TAT2 is diverted from the late secretory pathway to the vacuolar pathway. Furthermore, our results suggest that TAT2 stability and sorting are controlled by the TOR signaling pathway, and regulated inversely to that of GAP1.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-1423607, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-1715094, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-1718971, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-1791756, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-2125693, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-2194797, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-3054509, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-3131018, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-3552672, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-3552673, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-3905514, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-5474888, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7523855, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7528205, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7593183, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7606777, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7687745, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7706396, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7720726, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7798155, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-7888172, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8186460, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8247125, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8380177, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8387896, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8565073, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8596462, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8631913, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8657109, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8730101, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8741837, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8756348, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8909535, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-8943012, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9038344, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9045811, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9119204, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9199164, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9224897, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9343384, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9348664, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9409540, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9409822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9461583, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9489675, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9539725, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9545237, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9548714, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9573211, http://linkedlifedata.com/resource/pubmed/commentcorrection/10491387-9614172
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1227-38
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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