Linked Life Data

10488147

Source:http://linkedlifedata.com/resource/pubmed/id/10488147

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
39
pubmed:dateCreated
1999-11-4
pubmed:databankReference
pubmed:abstractText
Previously, we mapped the novel CC chemokine myeloid progenitor inhibitory factor 2 (MPIF-2)/eotaxin-2 to chromosome 7q11.23 (Nomiyama, H., Osborne, L. R., Imai, T., Kusuda, J., Miura, R., Tsui, L.-C., and Yoshie, O. (1998) Genomics 49, 339-340). Since chemokine genes tend to be clustered, unknown chemokines may be present in the vicinity of those mapped to new chromosomal loci. Prompted by this hypothesis, we analyzed the genomic region containing the gene for MPIF-2/eotaxin-2 (SCYA24) and have identified a novel CC chemokine termed eotaxin-3. The genes for MPIF-2/eotaxin-2 (SCYA24) and eotaxin-3 (SCYA26) are localized within a region of approximately 40 kilobases. By Northern blot analysis, eotaxin-3 mRNA was constitutively expressed in the heart and ovary. We have generated recombinant eotaxin-3 in a baculovirus expression system. Eotaxin-3 induced transient calcium mobilization specifically in CC chemokine receptor 3 (CCR3)-expressing L1.2 cells with an EC(50) of 3 nM. Eotaxin-3 competed the binding of (125)I-eotaxin to CCR3-expressing L1.2 cells with an IC(50) of 13 nM. Eotaxin-3 was chemotactic for normal peripheral blood eosinophils and basophils at high concentrations. Collectively, eotaxin-3 is yet another functional ligand for CCR3. The potency of eotaxin-3 as a CCR3 ligand seems, however, to be approximately 10-fold less than that of eotaxin. Identification of eotaxin-3 will further promote our understanding of the control of eosinophil trafficking and other CCR3-mediated biological phenomena. The strategy used in this study may also be applicable to identification of other unknown chemokine genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27975-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10488147-Amino Acid Sequence, pubmed-meshheading:10488147-Animals, pubmed-meshheading:10488147-Base Sequence, pubmed-meshheading:10488147-Cell Line, pubmed-meshheading:10488147-Chemokine CCL24, pubmed-meshheading:10488147-Chemokines, CC, pubmed-meshheading:10488147-Chemotactic Factors, pubmed-meshheading:10488147-Chromosome Mapping, pubmed-meshheading:10488147-Chromosomes, Human, Pair 7, pubmed-meshheading:10488147-Cloning, Molecular, pubmed-meshheading:10488147-Evolution, Molecular, pubmed-meshheading:10488147-Humans, pubmed-meshheading:10488147-Mice, pubmed-meshheading:10488147-Molecular Sequence Data, pubmed-meshheading:10488147-Multigene Family, pubmed-meshheading:10488147-Phylogeny, pubmed-meshheading:10488147-Receptors, CCR3, pubmed-meshheading:10488147-Receptors, Chemokine, pubmed-meshheading:10488147-Recombinant Proteins, pubmed-meshheading:10488147-Restriction Mapping, pubmed-meshheading:10488147-Transfection
pubmed:year
1999
pubmed:articleTitle
Molecular cloning of a novel human CC chemokine (Eotaxin-3) that is a functional ligand of CC chemokine receptor 3.
pubmed:affiliation
Shionogi Institute for Medical Science, Osaka 566-0022, Kinki University School of Medicine, Osaka 589-8511, Japan.
pubmed:publicationType
Journal Article