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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
39
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pubmed:dateCreated |
1999-11-4
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pubmed:abstractText |
Munc13-1 and DOC2 have been implicated in the regulation of exocytosis. Here we demonstrate in vivo that these two proteins undergo a transient phorbol ester-mediated and protein kinase C-independent interaction, resulting in the translocation of DOC2 from a vesicular localization to the plasma membrane. The translocation of DOC2 is dependent upon the DOC2 Munc interacting domain that binds specifically to Munc13-1, whereas the association of DOC2 with intracellular membranes is dependent on its C2 domains. This is the first direct in vivo demonstration of a protein-protein interaction between two presynaptic proteins and may represent a molecular basis for phorbol ester-dependent enhancement of exocytosis.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Benzophenanthridines,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DOC2A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DOC2B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Doc2a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Doc2a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phenanthridines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/UNC13B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Unc13b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Unc13h1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/chelerythrine
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27347-50
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10488064-Alkaloids,
pubmed-meshheading:10488064-Animals,
pubmed-meshheading:10488064-Benzophenanthridines,
pubmed-meshheading:10488064-Brain,
pubmed-meshheading:10488064-Calcium-Binding Proteins,
pubmed-meshheading:10488064-Cell Line,
pubmed-meshheading:10488064-Cell Membrane,
pubmed-meshheading:10488064-Enzyme Inhibitors,
pubmed-meshheading:10488064-Exocytosis,
pubmed-meshheading:10488064-Green Fluorescent Proteins,
pubmed-meshheading:10488064-Humans,
pubmed-meshheading:10488064-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10488064-Luminescent Proteins,
pubmed-meshheading:10488064-Mice,
pubmed-meshheading:10488064-Nerve Tissue Proteins,
pubmed-meshheading:10488064-Open Reading Frames,
pubmed-meshheading:10488064-Phenanthridines,
pubmed-meshheading:10488064-Protein Kinase C,
pubmed-meshheading:10488064-Rats,
pubmed-meshheading:10488064-Recombinant Fusion Proteins,
pubmed-meshheading:10488064-Tetradecanoylphorbol Acetate,
pubmed-meshheading:10488064-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Transient, phorbol ester-induced DOC2-Munc13 interactions in vivo.
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pubmed:affiliation |
Membrane Biology Group, Department of Biomedical Sciences, University of Edinburgh Medical School, Edinburgh EH8 9AG, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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