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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-10-21
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pubmed:abstractText |
The second major allergen of Chamaecyparis obtusa (Japanese cypress) pollen, Cha o 2, has been purified and its cDNA cloned. Of patients with pollinosis caused by C. obtusa, 82.5% produce IgE antibodies which react with purified Cha o 2. The purified protein has a molecular mass of 46 kDa and its 12 N-terminal amino acid sequence displays a high homology with that of Cry j 2, the second major allergen of Cryptomeria japonica pollen. cDNA clones coding for Cha o 2 have been isolated using Cry j 2 cDNA as a probe. Cha o 2 cDNA clones were sequenced and found to code a putative 50-residue signal sequence and a 464-residue mature protein with a molecular weight of 50 kDa. Two possible N-linked glycosylation sites were found in the sequence. The deduced amino acid sequence of Cha o 2 shows 74.3% identity with that of Cry j 2. In its primary structure, Cha o 2 shows significant identity with those of the polygalacturonases of avocado, tomato, and maize as well as Cry j 2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
166-71
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:10486272-Allergens,
pubmed-meshheading:10486272-Amino Acid Sequence,
pubmed-meshheading:10486272-Antibody Specificity,
pubmed-meshheading:10486272-Base Sequence,
pubmed-meshheading:10486272-Case-Control Studies,
pubmed-meshheading:10486272-Cloning, Molecular,
pubmed-meshheading:10486272-DNA, Complementary,
pubmed-meshheading:10486272-DNA, Plant,
pubmed-meshheading:10486272-Humans,
pubmed-meshheading:10486272-Immunoglobulin E,
pubmed-meshheading:10486272-Molecular Sequence Data,
pubmed-meshheading:10486272-Molecular Weight,
pubmed-meshheading:10486272-Plant Proteins,
pubmed-meshheading:10486272-Pollen,
pubmed-meshheading:10486272-Rhinitis, Allergic, Seasonal,
pubmed-meshheading:10486272-Sequence Homology, Amino Acid,
pubmed-meshheading:10486272-Trees
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pubmed:year |
1999
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pubmed:articleTitle |
Purification, identification, and cDNA cloning of Cha o 2, the second major allergen of Japanese cypress pollen.
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pubmed:affiliation |
Meiji Institute of Health Science, 540 Naruda, Odawara, Kanagawa, 250-0862, Japan.
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pubmed:publicationType |
Journal Article
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