Source:http://linkedlifedata.com/resource/pubmed/id/10485322
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1999-10-18
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pubmed:abstractText |
Working with Mel-85 (a human melanoma cell line), we have been able to detect a laminin-binding molecule with an apparent molecular mass of 100/110 kDa (Mel-85-LBM). Reduction with beta-mercaptoethanol decreases its molecular mass but does not affect its ability to bind laminin. This laminin interaction seems to be very specific since Mel-85-LBM binds laminin, but not fibronectin, vitronectin or type I collagen in affinity chromatography experiments. The molecule has a negative net charge at physiological pH and binds laminin in a divalent cation dependent way. Mel-85-LBM was metabolically radiolabeled with sodium [35S]-sulfate and chemical beta-elimination of purified Mel-85-LBM releases chondroitin sulfate chains. Mel-85-LBM is also sensitive to chondroitinase ABC digestion. These findings show that this molecule is a chondroitin sulfate proteoglycan. The location of this proteoglycan at the cell surface is evidenced by experiments using a polyclonal antiserum raised against purified Mel-85-LBM, that specifically reacts with just one molecule by western blotting among Mel-85 total cell extract as well as produces a positive signal by flow cytometry and a fluorescence profile of Mel-85 cells adhered on laminin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0300-8177
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
197
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39-48
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10485322-Cell Adhesion,
pubmed-meshheading:10485322-Cell Membrane,
pubmed-meshheading:10485322-Cell Movement,
pubmed-meshheading:10485322-Chondroitin Sulfate Proteoglycans,
pubmed-meshheading:10485322-Chromatography, Affinity,
pubmed-meshheading:10485322-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10485322-Humans,
pubmed-meshheading:10485322-Laminin,
pubmed-meshheading:10485322-Melanoma,
pubmed-meshheading:10485322-Protein Binding,
pubmed-meshheading:10485322-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Presence of a laminin-binding chondroitin sulfate proteoglycan at the cell surface of a human melanoma cell Mel-85.
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pubmed:affiliation |
Ludwig Institute for Cancer Research, São Paulo, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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