Linked Life Data

10484737

Source:http://linkedlifedata.com/resource/pubmed/id/10484737

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1999-9-3
pubmed:abstractText
The yellow-legged hornet, Vespa verutina, is widely distributed in both the mountain area and the suburbs of Taiwan and possesses highly toxic venom (LD50=0.02 microl/g mouse). By gel filtration on Fractogel (TSK HW 50f) followed by cation-exchange chromatography on Mono S column, three toxins designated as verutoxin 1, 2a and 2b (VT-1, VT-2a and VT-2b) were isolated from the venom. The toxin VT-1 had a molecular mass of 34,982 Da and an LD50 value of 3.61 microg/g mouse. Toxin VT-2a and 2b were more basic and more toxic than VT-1. VT-2a and 2b were isotoxins with molecular masses differing in only 14 Da (33,360 and 33,374 Da, respectively) and had a similar toxicity in mice (LD50=0.87 microg/g mouse). All three toxins were capable of catalyzing the hydrolysis of emulsified phospholipids and lysophosphatide, but not sphingomyelin. Analysis of the hydrolyzed products (fatty acid and lyso-compound) by a liquid chromatography/mass spectrometer revealed that the toxins liberates fatty acid mainly from the 1-position of the synthetic phospholipid. This result indicates that verutoxins possess phospholipase A1 activity. Toxin VT-1 showed higher phospholipase activity than VT-2a and 2b. However, the latter toxins exhibited much higher direct hemolytic activity toward the mouse red blood cells. Vespid phospholipases are known as one of the three major venom allergens in many species of wasps. Our studies indicate that vespid phospholipases A1, in addition to acting as allergens, possess direct toxic actions that may also cause death in animals. Toxin VT-2a and 2b which possess potent hemolytic activity and high lethality in mice may act as the lethal factor of V. verutina venom.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0041-0101
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1015-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Three toxins with phospholipase activity isolated from the yellow-legged hornet (Vespa verutina) venom.
pubmed:affiliation
Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't