Linked Life Data

10484348

Source:http://linkedlifedata.com/resource/pubmed/id/10484348

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
1999-10-28
pubmed:abstractText
Recently three equations for estimating gluconeogenesis in vivo have been proposed, two by J. A. Tayek and J. Katz [Am. J. Physiol. 270 (Endocrinol. Metab. 33): E709-E717, 1996, and Am. J. Physiol. 272 (Endocrinol. Metab. 35): E476-E484, 1997] and one by B. R. Landau, J. Wahren, K. Ekberg, S. F. Previs, D. Yang, and H. Brunengraber [Am. J. Physiol. 274 (Endocrinol. Metab. 37): E954-E961, 1998]. Both groups estimate gluconeogenesis from cycling of [U-13C]glucose to lactate and back to glucose, detected by mass spectrometry. Landau's approach is based on analysis of labeled molecules, whereas Tayek and Katz's is based on labeling of carbon atoms by use of the concept of "molar enrichment," which weights each mass isotopomer by the number of labeled carbons. We derived an equation very similar to Landau's using binomial probability. Our analysis demonstrates that the molecular-based approach is correct. Additionally, equations appropriate for 14C studies are not appropriate for 13C studies, because the method used to detect 14C, decay of atoms, differs from 13C mass isotopomers detected as labeled molecules. We conclude that the molar enrichment carbon-based approach is not useful in the derivation of equations for the polymerization of molecules detected by mass spectrometry of molecules, and we confirm the findings of Landau et al.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E395-400
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Estimating gluconeogenesis with [U-13C]glucose: molecular condensation requires a molecular approach.
pubmed:affiliation
Department of Physiology, The George Washington University School of Medicine and Health Sciences, Washington, District of Columbia 20037, USA. phyjkk@gwumc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review