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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-11-8
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pubmed:abstractText |
Proteases secreted by Aspergillus fumigatus induce the production of cytokines by epithelial cells, including interleukin (IL)-6 and IL-8. In the present study, we focused on the mechanism(s) by which A. fumigatus-derived proteases elicit cytokine production in epithelial cells. In the epithelial cell line A549, IL-6 and IL-8 mRNA levels were enhanced by proteases as a result of transcriptional induction of the respective genes. Transcriptional induction of both genes coincided with enhanced DNA binding of nuclear factor (NF)-kappaB and NF-IL6, whereas activator protein-1 was unlikely to be involved. The enhanced transcriptional activity could be inhibited by the addition of chymostatin, showing serine protease dependency. Posttranscriptional mechanisms affecting the stability of IL-6 and IL-8 mRNAs were not involved in protease-induced IL-6 and IL-8 production. These data show that after exposure to A. fumigatus-derived proteases, IL-6 and IL-8 gene expressions are up-regulated as a result of transcriptional mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protease Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1267-74
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10479157-Aspergillus fumigatus,
pubmed-meshheading:10479157-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10479157-DNA-Binding Proteins,
pubmed-meshheading:10479157-Endopeptidases,
pubmed-meshheading:10479157-Epithelial Cells,
pubmed-meshheading:10479157-Gene Expression Regulation,
pubmed-meshheading:10479157-Humans,
pubmed-meshheading:10479157-Interleukin-6,
pubmed-meshheading:10479157-Interleukin-8,
pubmed-meshheading:10479157-Kinetics,
pubmed-meshheading:10479157-Lung Neoplasms,
pubmed-meshheading:10479157-NF-kappa B,
pubmed-meshheading:10479157-Nuclear Proteins,
pubmed-meshheading:10479157-Polymerase Chain Reaction,
pubmed-meshheading:10479157-Protease Inhibitors,
pubmed-meshheading:10479157-RNA, Messenger,
pubmed-meshheading:10479157-Transcription, Genetic,
pubmed-meshheading:10479157-Transcription Factor AP-1,
pubmed-meshheading:10479157-Transcription Factors,
pubmed-meshheading:10479157-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Proteases from Aspergillus fumigatus induce interleukin (IL)-6 and IL-8 production in airway epithelial cell lines by transcriptional mechanisms.
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pubmed:affiliation |
Laboratory of Allergology & Pulmonology, Department of Pathology, University Hospital Groningen, 9713 GZ Groningen, The Netherlands. P.Borger@path.azg.nl
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pubmed:publicationType |
Journal Article
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