Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-10-12
pubmed:abstractText
The present study was designed to test the concept that platelets release a humoral factor that plays a regulatory role in megakaryopoiesis. The results showed that, among various hematoregulatory cytokines examined, transforming growth factor-beta1 (TGF-beta1) was by far the most potent enhancer of mRNA expression of bone marrow stromal thrombopoietin (TPO), a commitment of lineage specificity. The TPO, in turn, induced TGF-beta receptors I and II on megakaryoblasts at the midmegakaryopoietic stage; at this stage, TGF-beta1 was able to arrest the maturation of megakaryocyte colony-forming units (CFU-Meg). This effect was relatively specific when compared with its effect on burst-forming unit-erythroid (BFU-E) or colony-forming unit-granulocyte-macrophage (CFU-GM). In patients with idiopathic thrombocytopenic purpura (ITP), the levels of both TGF-beta1 and stromal TPO mRNA were correlatively increased and an arrest of megakaryocyte maturation was observed. These in vivo findings are in accord with the aforementioned in vitro results. Thus, the results of the present investigation suggest that TGF-beta1 is one of the pathophysiological feedback regulators of megakaryopoiesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1961-70
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10477725-Bone Marrow, pubmed-meshheading:10477725-Colony-Forming Units Assay, pubmed-meshheading:10477725-Erythropoietin, pubmed-meshheading:10477725-Gene Expression Regulation, pubmed-meshheading:10477725-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:10477725-Hematopoiesis, pubmed-meshheading:10477725-Humans, pubmed-meshheading:10477725-Interleukin-3, pubmed-meshheading:10477725-Interleukin-6, pubmed-meshheading:10477725-Megakaryocytes, pubmed-meshheading:10477725-Models, Biological, pubmed-meshheading:10477725-Purpura, Thrombocytopenic, pubmed-meshheading:10477725-RNA, Messenger, pubmed-meshheading:10477725-Recombinant Proteins, pubmed-meshheading:10477725-Reference Values, pubmed-meshheading:10477725-Stromal Cells, pubmed-meshheading:10477725-Thrombopoietin, pubmed-meshheading:10477725-Transcription, Genetic, pubmed-meshheading:10477725-Transforming Growth Factor beta, pubmed-meshheading:10477725-Tumor Necrosis Factor-alpha
pubmed:year
1999
pubmed:articleTitle
Transforming growth factor-beta1 (TGF-beta1) induces thrombopoietin from bone marrow stromal cells, which stimulates the expression of TGF-beta receptor on megakaryocytes and, in turn, renders them susceptible to suppression by TGF-beta itself with high specificity.
pubmed:affiliation
4th Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't