| pubmed-article:10477616 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C0023516 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C0271510 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C1416487 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C1511545 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:10477616 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:10477616 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10477616 | pubmed:dateCreated | 1999-10-4 | lld:pubmed |
| pubmed-article:10477616 | pubmed:abstractText | IL-4 is known to induce recruitment of eosinophils and mononuclear leukocytes. In vitro this occurs in part by selective expression of VCAM-1, the ligand for the alpha 4 integrin. The objective of this study was to determine the molecular mechanisms that underlie IL-4-induced leukocyte recruitment in vivo. Mice received an intrascrotal injection of IL-4 (100 ng). Twenty-four hours later, leukocyte rolling, adhesion, and emigration in cremasteric postcapillary venules were examined via intravital microscopy, and expression of VCAM-1 and P- and E-selectin was quantitated using a radiolabeled mAb technique. IL-4 increased VCAM-1 expression, but P-selectin and E-selectin remained at constitutive levels. IL-4 induced significant increases in leukocyte adhesion and emigration, with 50% of the emigrated cells being eosinophils and the remainder being mononuclear leukocytes. Leukocyte rolling in IL-4-treated mice was >95% inhibitable using an anti-P-selectin Ab. However, IL-4-induced leukocyte recruitment was unaltered in mice treated chronically with P-selectin Ab or mice deficient in either P-selectin or P- and E-selectin, suggesting that the residual rolling supported all of the IL-4-induced recruitment. In IL-4-treated mice following P-selectin blockade, tethering and rolling were not dependent on L-selectin, but were abolished by alpha 4 integrin blockade. These findings show that the alpha 4 integrin can initiate leukocyte-endothelial cell interactions in the absence of selectins under shear conditions in vivo, and that the absence of selectins does not affect recruitment of eosinophils and mononuclear cells to IL-4-treated tissue. | lld:pubmed |
| pubmed-article:10477616 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477616 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10477616 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477616 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:10477616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477616 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10477616 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10477616 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:10477616 | pubmed:author | pubmed-author:GrangerD NDN | lld:pubmed |
| pubmed-article:10477616 | pubmed:author | pubmed-author:KubesPP | lld:pubmed |
| pubmed-article:10477616 | pubmed:author | pubmed-author:HickeyM JMJ | lld:pubmed |
| pubmed-article:10477616 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10477616 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10477616 | pubmed:volume | 163 | lld:pubmed |
| pubmed-article:10477616 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10477616 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10477616 | pubmed:pagination | 3441-8 | lld:pubmed |
| pubmed-article:10477616 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:10477616 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10477616 | pubmed:articleTitle | Molecular mechanisms underlying IL-4-induced leukocyte recruitment in vivo: a critical role for the alpha 4 integrin. | lld:pubmed |
| pubmed-article:10477616 | pubmed:affiliation | Immunology Research Group, University of Calgary, Alberta, Canada. | lld:pubmed |
| pubmed-article:10477616 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10477616 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10477616 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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