Linked Life Data

10477616

Source:http://linkedlifedata.com/resource/pubmed/id/10477616

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-10-4
pubmed:abstractText
IL-4 is known to induce recruitment of eosinophils and mononuclear leukocytes. In vitro this occurs in part by selective expression of VCAM-1, the ligand for the alpha 4 integrin. The objective of this study was to determine the molecular mechanisms that underlie IL-4-induced leukocyte recruitment in vivo. Mice received an intrascrotal injection of IL-4 (100 ng). Twenty-four hours later, leukocyte rolling, adhesion, and emigration in cremasteric postcapillary venules were examined via intravital microscopy, and expression of VCAM-1 and P- and E-selectin was quantitated using a radiolabeled mAb technique. IL-4 increased VCAM-1 expression, but P-selectin and E-selectin remained at constitutive levels. IL-4 induced significant increases in leukocyte adhesion and emigration, with 50% of the emigrated cells being eosinophils and the remainder being mononuclear leukocytes. Leukocyte rolling in IL-4-treated mice was >95% inhibitable using an anti-P-selectin Ab. However, IL-4-induced leukocyte recruitment was unaltered in mice treated chronically with P-selectin Ab or mice deficient in either P-selectin or P- and E-selectin, suggesting that the residual rolling supported all of the IL-4-induced recruitment. In IL-4-treated mice following P-selectin blockade, tethering and rolling were not dependent on L-selectin, but were abolished by alpha 4 integrin blockade. These findings show that the alpha 4 integrin can initiate leukocyte-endothelial cell interactions in the absence of selectins under shear conditions in vivo, and that the absence of selectins does not affect recruitment of eosinophils and mononuclear cells to IL-4-treated tissue.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3441-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10477616-Animals, pubmed-meshheading:10477616-Antigens, CD, pubmed-meshheading:10477616-Cell Adhesion, pubmed-meshheading:10477616-Cell Movement, pubmed-meshheading:10477616-E-Selectin, pubmed-meshheading:10477616-Eosinophils, pubmed-meshheading:10477616-Injections, Intradermal, pubmed-meshheading:10477616-Integrin alpha4, pubmed-meshheading:10477616-Integrins, pubmed-meshheading:10477616-Interleukin-4, pubmed-meshheading:10477616-Leukocytes, Mononuclear, pubmed-meshheading:10477616-Male, pubmed-meshheading:10477616-Mice, pubmed-meshheading:10477616-Mice, Inbred C57BL, pubmed-meshheading:10477616-Mice, Knockout, pubmed-meshheading:10477616-Microcirculation, pubmed-meshheading:10477616-Microscopy, Video, pubmed-meshheading:10477616-P-Selectin, pubmed-meshheading:10477616-Vascular Cell Adhesion Molecule-1
pubmed:year
1999
pubmed:articleTitle
Molecular mechanisms underlying IL-4-induced leukocyte recruitment in vivo: a critical role for the alpha 4 integrin.
pubmed:affiliation
Immunology Research Group, University of Calgary, Alberta, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't