Linked Life Data

10477594

Source:http://linkedlifedata.com/resource/pubmed/id/10477594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-10-4
pubmed:abstractText
Two types of dendritic cells (DC) are circulating in human blood and can be identified by their differential expression of the myeloid Ag CD11c. In this study, we show that CD11c- peripheral blood (PB)-DC correspond to plasmacytoid DC of lymphoid tissue not only by their surface Ag expression profile but, more impressively, by their peculiar ultramorphology. We also demonstrate that CD11c- and CD11c+ DC differ in the quality of their response to and in their requirement for certain cytokines. Freshly isolated CD11c- cells depend on IL-3 for survival and use autocrine or exogenous TNF-alpha as maturation signal, leading to the appearance of a highly dendritic phenotype, the up-regulation and redistribution of MHC class II from lysosomal compartments to the plasma membrane, the increased expression of costimulatory molecules, and the switch from a high Ag-processing to a low Ag-processing/potent accessory cell mode. Surprisingly, IL-4 efficiently killed freshly isolated CD11c- PB-DC, but did not impair the viability of CD11c+ PB-DC and, together with GM-CSF, induced maturation of these cells. A direct functional comparison revealed that neo-Ag-modified and subsequently matured CD11c- but to a lesser extent CD11c+ DC were able to prime naive Ag-specific CD4+ T cells. Our findings show that two diverse DC types respond to certain T cell-derived cytokines in a differential manner and, thus, suggest that suppression or activation of functionally diverse DC types may be a novel mechanism for the regulation of the quantity and quality of immune responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3250-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10477594-Animals, pubmed-meshheading:10477594-Antigen Presentation, pubmed-meshheading:10477594-Antigens, CD4, pubmed-meshheading:10477594-CD4-Positive T-Lymphocytes, pubmed-meshheading:10477594-CD8-Positive T-Lymphocytes, pubmed-meshheading:10477594-Cell Differentiation, pubmed-meshheading:10477594-Cell Lineage, pubmed-meshheading:10477594-Cell Separation, pubmed-meshheading:10477594-Cell Survival, pubmed-meshheading:10477594-Cells, Cultured, pubmed-meshheading:10477594-Cytokines, pubmed-meshheading:10477594-Dendritic Cells, pubmed-meshheading:10477594-Down-Regulation, pubmed-meshheading:10477594-Epitopes, T-Lymphocyte, pubmed-meshheading:10477594-Growth Substances, pubmed-meshheading:10477594-Hemocyanin, pubmed-meshheading:10477594-Humans, pubmed-meshheading:10477594-Immunophenotyping, pubmed-meshheading:10477594-Integrin alphaXbeta2, pubmed-meshheading:10477594-Isoantigens, pubmed-meshheading:10477594-Lymphocyte Activation, pubmed-meshheading:10477594-Mollusca, pubmed-meshheading:10477594-Plasma Cells, pubmed-meshheading:10477594-Receptors, Cytokine, pubmed-meshheading:10477594-Signal Transduction
pubmed:year
1999
pubmed:articleTitle
Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines.
pubmed:affiliation
Division of Immunology, Department of Dermatology, University of Vienna Medical School, Austria.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't