Linked Life Data

10473624

Source:http://linkedlifedata.com/resource/pubmed/id/10473624

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
37
pubmed:dateCreated
1999-10-7
pubmed:abstractText
Two putative CCAAT/enhancer-binding protein (C/EBP) response elements were identified in the proximal promoter of the human steroidogenic acute regulatory protein (StAR) gene, which encodes a key protein-regulating steroid hormone synthesis. Expression of C/EBPalpha and -beta increased StAR promoter activity in COS-1 and HepG2 cells. Cotransfection of C/EBPalpha or -beta and steroidogenic factor 1, a transcription factor required for cAMP regulation of StAR expression, into COS-1 augmented 8-bromoadenosine 3':5'-cyclic monophosphate (8-Br-cAMP)-stimulated promoter activity. When the putative C/EBP response elements were mutated, individually or together, a pronounced decline in basal StAR promoter activity in human granulosa-lutein cells resulted, but the fold stimulation of promoter activity by 8-Br-cAMP was unaffected. Recombinant C/EBPalpha and -beta bound to the two identified sequences but not the mutated elements. Human granulosa-lutein cell nuclear extracts also bound these elements but not the mutated sequences. An antibody to C/EBPbeta, but not C/EBPalpha, supershifted the nuclear protein complex associated with the more distal element. The complex formed by nuclear extracts with the proximal element was not supershifted by either antibody. Western blot analysis revealed the presence of C/EBPalpha and C/EBPbeta in human granulosa-lutein cell nuclear extracts. C/EBPbeta levels were up-regulated 3-fold by 8-Br-cAMP treatment. Our studies demonstrate a role for C/EBPbeta as well as yet to be identified proteins, which can bind to C/EBP response elements, in the regulation of StAR gene expression and suggest a mechanism by which C/EBPbeta participates in the cAMP regulation of StAR gene transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26591-8
pubmed:dateRevised
2009-8-12
pubmed:meshHeading
pubmed-meshheading:10473624-Amino Acid Sequence, pubmed-meshheading:10473624-Animals, pubmed-meshheading:10473624-Blotting, Western, pubmed-meshheading:10473624-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:10473624-Cell Line, pubmed-meshheading:10473624-Cell Nucleus, pubmed-meshheading:10473624-DNA-Binding Proteins, pubmed-meshheading:10473624-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:10473624-Female, pubmed-meshheading:10473624-Gene Expression Regulation, pubmed-meshheading:10473624-Granulosa Cells, pubmed-meshheading:10473624-Humans, pubmed-meshheading:10473624-Molecular Sequence Data, pubmed-meshheading:10473624-Nuclear Proteins, pubmed-meshheading:10473624-Phosphoproteins, pubmed-meshheading:10473624-Promoter Regions, Genetic, pubmed-meshheading:10473624-Recombinant Proteins, pubmed-meshheading:10473624-Sequence Homology, Amino Acid, pubmed-meshheading:10473624-Steroidogenic Factor 1
pubmed:year
1999
pubmed:articleTitle
CCAAT/enhancer-binding proteins regulate expression of the human steroidogenic acute regulatory protein (StAR) gene.
pubmed:affiliation
Center for Research on Reproduction and Women's Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. lchriste@mail.med.upenn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't