Linked Life Data

10473579

Source:http://linkedlifedata.com/resource/pubmed/id/10473579

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
37
pubmed:dateCreated
1999-10-7
pubmed:databankReference
pubmed:abstractText
beta-Defensins are microbicidal peptides implicated in host defense functions of phagocytic leukocytes and certain surface epithelial cells. Here we investigated the genetic structures and cellular expression of BNBD-4, -12, and -13, three prototypic bovine neutrophil beta-defensins. Characterization of the corresponding cDNAs indicated that BNBD-4 (41 residues) derives from a 63-amino acid prepropeptide and that BNBD-12 (38 residues) and BNBD-13 (42 residues) derive from a common 60-amino acid precursor (BNBD-12/13). The peptides were found to be encoded by two-exon genes that are closely related to bovine epithelial beta-defensin genes. BNBD-4 and BNBD-12/13 mRNAs were most abundant in bone marrow, but were expressed differentially in certain non-myeloid tissues. In situ hybridization and immunohistochemical studies demonstrated that BNBD-4 synthesis is completed early in myelopoiesis. BNBD-12 was localized exclusively to the novel dense granules, organelles that also contain precursors of cathelicidins, antimicrobial peptides that undergo proteolytic processing during phagocytosis. In contrast to cathelicidins, Western blot analyses revealed that mature beta-defensins are the predominant organellar form in myeloid cells. Stimulation of neutrophils with phorbol myristate acetate induced secretion of BNBD-12, indicating that it is co-secreted with pro-cathelicidins. The exocytosis of BNBD-12 by activated neutrophils reveals different mobilization pathways for myeloid alpha- and beta-defensins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
26249-58
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10473579-Amino Acid Sequence, pubmed-meshheading:10473579-Animals, pubmed-meshheading:10473579-Base Sequence, pubmed-meshheading:10473579-Cattle, pubmed-meshheading:10473579-Cloning, Molecular, pubmed-meshheading:10473579-DNA, Complementary, pubmed-meshheading:10473579-Defensins, pubmed-meshheading:10473579-Exocytosis, pubmed-meshheading:10473579-Gene Expression, pubmed-meshheading:10473579-Microscopy, Electron, pubmed-meshheading:10473579-Molecular Sequence Data, pubmed-meshheading:10473579-Neutrophil Activation, pubmed-meshheading:10473579-Neutrophils, pubmed-meshheading:10473579-Protein Biosynthesis, pubmed-meshheading:10473579-Proteins, pubmed-meshheading:10473579-RNA, Messenger, pubmed-meshheading:10473579-Sequence Homology, Amino Acid, pubmed-meshheading:10473579-Tetradecanoylphorbol Acetate, pubmed-meshheading:10473579-beta-Defensins
pubmed:year
1999
pubmed:articleTitle
Cloning and expression of bovine neutrophil beta-defensins. Biosynthetic profile during neutrophilic maturation and localization of mature peptide to novel cytoplasmic dense granules.
pubmed:affiliation
Department of Pathology, College of Medicine, University of California, Irvine, California 92697, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't