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pubmed-article:10471587pubmed:abstractTextThe conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).lld:pubmed
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pubmed-article:10471587pubmed:articleTitleAntimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.lld:pubmed
pubmed-article:10471587pubmed:affiliationDivision of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom.lld:pubmed
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