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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-11-4
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pubmed:abstractText |
The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-1097546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-1373404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-2030670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-2126772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-2449348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-3840827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-3891709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-4002096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-4698212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-5413965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-6622887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-7085567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-7793888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-7806358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-8156277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-8162358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-8586701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-8722533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-8980763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10471587-9215095
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2311-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1999
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pubmed:articleTitle |
Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.
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pubmed:affiliation |
Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield S10 2RX, United Kingdom.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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