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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-10-19
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pubmed:abstractText |
Polyketide synthases (PKSs) are bacterial multienzyme systems that synthesize a broad range of natural products. The 'minimal' PKS consists of a ketosynthase, a chain length factor, an acyl carrier protein and a malonyl transferase. Auxiliary components (ketoreductases, aromatases and cyclases are involved in controlling the oxidation level and cyclization of the nascent polyketide chain. We describe the heterologous expression and reconstitution of several auxiliary PKS components including the actinorhodin ketoreductase (act KR), the griseusin aromatase/cyclase (gris ARO/CYC), and the tetracenomycin aromatase/cyclase (tcm ARO/CYC).
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acyl Carrier Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Acyl-Carrier Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Acyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde-Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid Synthetase Complex...,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fabD protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/polyketide synthase ketoreductase,
http://linkedlifedata.com/resource/pubmed/chemical/tetracenomycin F2 cyclase...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1074-5521
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
607-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10467128-Acyl Carrier Protein,
pubmed-meshheading:10467128-Acyl-Carrier Protein S-Malonyltransferase,
pubmed-meshheading:10467128-Acyltransferases,
pubmed-meshheading:10467128-Alcohol Oxidoreductases,
pubmed-meshheading:10467128-Aldehyde-Lyases,
pubmed-meshheading:10467128-Bacterial Proteins,
pubmed-meshheading:10467128-Chromatography, High Pressure Liquid,
pubmed-meshheading:10467128-Cross-Linking Reagents,
pubmed-meshheading:10467128-Cyclization,
pubmed-meshheading:10467128-Escherichia coli,
pubmed-meshheading:10467128-Escherichia coli Proteins,
pubmed-meshheading:10467128-Fatty Acid Synthetase Complex, Type II,
pubmed-meshheading:10467128-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10467128-Indicators and Reagents,
pubmed-meshheading:10467128-Kinetics,
pubmed-meshheading:10467128-Molecular Weight,
pubmed-meshheading:10467128-Multienzyme Complexes,
pubmed-meshheading:10467128-Mutation,
pubmed-meshheading:10467128-Recombinant Proteins,
pubmed-meshheading:10467128-Streptomyces
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pubmed:year |
1999
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pubmed:articleTitle |
Heterologous expression, purification, reconstitution and kinetic analysis of an extended type II polyketide synthase.
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pubmed:affiliation |
Department of Chemical Engineering, Stanford University, Stanford, California 94305-5025, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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