Linked Life Data

10467023

Source:http://linkedlifedata.com/resource/pubmed/id/10467023

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-10-21
pubmed:abstractText
To determine whether functional atypical beta-adrenoceptors (beta(3)-adrenoceptors) are present in pulmonary vascular smooth muscle, we studied isolated canine pulmonary arterial rings under isometric conditions in vitro. Addition of beta-adrenoceptor agonists produced a concentration-dependent relaxation of noradrenaline-precontracted tissues, a rank order potency being isoproterenol (1) > salbutamol (0.95) > selective beta(3)-adrenoceptor agonists, CL 316243 (0.85), and BRL 37344 (0. 83). A marked desensitization to salbutamol occurred by pretreatment with salbutamol but not with CL 316243. When beta(1)-adrenoceptors had been blocked, the relaxant responses to salbutamol were competitively antagonized by the beta(2)-adrenoceptor antagonist ICI 118551 with a pA(2) value of 7.67 +/- 0.21 (mean +/- S.E.), but the response to CL 316243 was weekly antagonized by ICI 118551 only at a high concentration of 10(-5) M, where an apparent pA(2) value was 5. 24. In contrast, cyanopindolol, a nonselective beta-adrenoceptor antagonist, antagonized CL 316243-induced relaxation in a competitive manner with a pA(2) of 6.10 +/- 0.11. This pA(2) value was lower than that when salbutamol was used as an agonist (6.69 +/- 0.14, p < 0.01). Intracellular 3',5'-cyclic adenosine monophosphate (cAMP) levels were increased by CL 316243 in a concentration-dependent fashion, an effect that was not altered by ICI 118551. These results suggest that beta(3)-adrenoceptors may exist in canine pulmonary artery smooth muscle and that stimulation of this atypical receptor causes vasodilation through a cAMP-dependent pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0341-2040
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Atypical adrenoceptor-mediated relaxation of canine pulmonary artery through a cyclic adenosine monophosphate-dependent pathway.
pubmed:affiliation
First Department of Medicine, Tokyo Women's Medical College, Tokyo 162, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't