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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1999-11-24
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pubmed:abstractText |
Binding of Zn(II), Cu(II) and Fe(II) ions to A beta1-40, A beta1-42 and a single tryptophan mutant of Abeta 1-40 in solution at pH 7.4 was studied by fluorescent titration. Job plots and fitting of titration curves revealed formation of 1:1 and 1:2 peptide-metal complexes. For dimeric peptides A beta1-40 and A betaF4W the order of metal to peptide affinities is Fe < Cu > Zn, which is in agreement with the Irving-Williams series of complex stability. The affinity of A beta1-42 for Fe increases dramatically upon aggregation: K(D) changes from ca. 100 to ca. 0.2 microM.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0960-894X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2243-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10465554-Alzheimer Disease,
pubmed-meshheading:10465554-Amyloid beta-Peptides,
pubmed-meshheading:10465554-Binding, Competitive,
pubmed-meshheading:10465554-Copper,
pubmed-meshheading:10465554-Humans,
pubmed-meshheading:10465554-Iron,
pubmed-meshheading:10465554-Peptide Fragments,
pubmed-meshheading:10465554-Spectrometry, Fluorescence,
pubmed-meshheading:10465554-Zinc
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pubmed:year |
1999
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pubmed:articleTitle |
Binding of Zn(II), Cu(II), and Fe(II) ions to Alzheimer's A beta peptide studied by fluorescence.
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pubmed:affiliation |
Department of Molecular Biology and Biochemistry, University of California at Irvine, 92697, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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