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rdf:type |
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lifeskim:mentions |
umls-concept:C0006063,
umls-concept:C0006556,
umls-concept:C0017262,
umls-concept:C0026809,
umls-concept:C0033684,
umls-concept:C0042776,
umls-concept:C0086022,
umls-concept:C0086312,
umls-concept:C0205263,
umls-concept:C0206243,
umls-concept:C0301872,
umls-concept:C0442335,
umls-concept:C0950111,
umls-concept:C1171362,
umls-concept:C1512977,
umls-concept:C1515670,
umls-concept:C1538092,
umls-concept:C1705099,
umls-concept:C1860991
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pubmed:issue |
4
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pubmed:dateCreated |
1999-10-5
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pubmed:abstractText |
Bovine viral diarrhea virus (BVDV) is a primary pathogen responsible for bovine enteric, respiratory and reproductive failure. A genetic region is encoding the p80 (NS3) of BVDV as the most conserved protein among Pestiviruses. BVDV infection in cattle induces NS3 specific lymphocyte proliferation and humoral responses. To generate a DNA vaccine against BVDV, the gene for BVDV-NADL NS3 was cloned into an eukaryotic expression vector of Semiliki Forest virus (pSFV-1). Quadriceps muscles of BALB/c mice were injected with recombinant DNA generated statistically significant cytotoxic T-lymphocyte activity (CTL) and cell mediated immune (CMI) responses against cytopathic and noncytopathic BVDV. Whereas, the BVDV-NS3 did not generate neutralizing antibodies against BVDVin mice. pSFV-1-NS3 DNA was subjected to in vitro transcription into mRNA. The mRNA was transfected into baby hamster kidney cells (BHK-21) and Madin-Darby bovine kidney cells (MDBK). The recombinant cells were used in the detection of DNA antigen responses by immunological assays. This report establishes the ability of BVDV-NS3 DNA inoculation to induce a strong cellular immune responses in mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/p80 protein, bovine viral diarrhea...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0147-9571
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-46
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10465327-Animals,
pubmed-meshheading:10465327-Antibodies, Viral,
pubmed-meshheading:10465327-Antibody Formation,
pubmed-meshheading:10465327-Bovine Virus Diarrhea-Mucosal Disease,
pubmed-meshheading:10465327-Cattle,
pubmed-meshheading:10465327-Cell Line,
pubmed-meshheading:10465327-Cricetinae,
pubmed-meshheading:10465327-DNA, Complementary,
pubmed-meshheading:10465327-Genetic Vectors,
pubmed-meshheading:10465327-Mice,
pubmed-meshheading:10465327-Mice, Inbred BALB C,
pubmed-meshheading:10465327-Peptide Hydrolases,
pubmed-meshheading:10465327-RNA Helicases,
pubmed-meshheading:10465327-Semliki forest virus,
pubmed-meshheading:10465327-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10465327-Transfection,
pubmed-meshheading:10465327-Vaccines, DNA,
pubmed-meshheading:10465327-Viral Nonstructural Proteins,
pubmed-meshheading:10465327-Viral Vaccines
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pubmed:year |
1999
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pubmed:articleTitle |
Semiliki forest virus vector carrying the bovine viral diarrhea virus NS3 (p80) cDNA induced immune responses in mice and expressed BVDV protein in mammalian cells.
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pubmed:affiliation |
JN-International, Inc, Oakland, NE 68045, USA. jnii@trsonline.net
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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