| pubmed-article:10465294 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10465294 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10465294 | lifeskim:mentions | umls-concept:C0021745 | lld:lifeskim |
| pubmed-article:10465294 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:10465294 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10465294 | lifeskim:mentions | umls-concept:C1414674 | lld:lifeskim |
| pubmed-article:10465294 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:10465294 | pubmed:dateCreated | 1999-9-9 | lld:pubmed |
| pubmed-article:10465294 | pubmed:abstractText | Tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) are cytokines that can individually or additively suppress thyroid cell function and the expression of thyroid-specific genes, such as thyroglobulin (TG) and thyroperoxidase (TPO). Thyroid transcription factor-2 (TTF-2) is a DNA-binding protein that modulates the expression of TG and TPO genes. In the present study, we examine the effects of TNF-alpha and IFN-gamma on TTF-2 gene expression, as well as the DNA-binding activity of TTF-2. FRTL-5 cells were maintained in 5H medium containing 0.2% calf serum for 7 days, then incubated with TNF-alpha, IFN-gamma, or TNF-alpha plus IFN-gamma. Total RNA was isolated and Northern blotted. TNF-alpha (50 ng/ml) only slightly suppressed (61+/-2% compared with control), whereas IFN-gamma (100 U/ml) modestly decreased TTF-2 messenger RNA (mRNA) levels (34+/-4%). TNF-alpha and IFN-gamma simultaneously caused a marked decrease in TTF-2 mRNA levels (13+/-2%). The suppressive effects of TNF-alpha and IFN-gamma on TTF-2 mRNA levels were concentration dependent and maximal at 50 ng/ml TNF-alpha with 100 U/ml IFN-gamma. The suppressive effect was also time dependent, reaching a maximum 12 h after exposure. Moreover, the suppressive effects of TNF-alpha and IFN-gamma upon rat TG and TTF-2 mRNA levels were similar. To test whether TNF-alpha and IFN-gamma alter TTF-2-binding to DNA, we performed electrophoretic mobility shift assays using a TTF-2-binding element in the rat TG gene as a probe. Formation of the TTF-2/DNA complex was decreased by TNF-alpha and/or IFN-gamma. Our results demonstrate that TNF-alpha and IFN-gamma additively reduce the gene expression and DNA-binding of TTF-2. These data suggest that TTF-2 is involved in the TNF-alpha and IFN-gamma-induced suppression of thyroid-specific gene expression. | lld:pubmed |
| pubmed-article:10465294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10465294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10465294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10465294 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10465294 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:10465294 | pubmed:author | pubmed-author:EndoTT | lld:pubmed |
| pubmed-article:10465294 | pubmed:author | pubmed-author:OnayaTT | lld:pubmed |
| pubmed-article:10465294 | pubmed:author | pubmed-author:ShimuraHH | lld:pubmed |
| pubmed-article:10465294 | pubmed:author | pubmed-author:MiyazakiAA | lld:pubmed |
| pubmed-article:10465294 | pubmed:author | pubmed-author:HaraguchiKK | lld:pubmed |
| pubmed-article:10465294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10465294 | pubmed:volume | 140 | lld:pubmed |
| pubmed-article:10465294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10465294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10465294 | pubmed:pagination | 4214-20 | lld:pubmed |
| pubmed-article:10465294 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:10465294 | pubmed:meshHeading | pubmed-meshheading:10465294... | lld:pubmed |
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| pubmed-article:10465294 | pubmed:meshHeading | pubmed-meshheading:10465294... | lld:pubmed |
| pubmed-article:10465294 | pubmed:meshHeading | pubmed-meshheading:10465294... | lld:pubmed |
| pubmed-article:10465294 | pubmed:meshHeading | pubmed-meshheading:10465294... | lld:pubmed |
| pubmed-article:10465294 | pubmed:meshHeading | pubmed-meshheading:10465294... | lld:pubmed |
| pubmed-article:10465294 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10465294 | pubmed:articleTitle | Tumor necrosis factor-alpha and interferon-gamma suppress both gene expression and deoxyribonucleic acid-binding of TTF-2 in FRTL-5 cells. | lld:pubmed |
| pubmed-article:10465294 | pubmed:affiliation | Third Department of Internal Medicine, Yamanashi Medical University, Tamaho, Japan. | lld:pubmed |
| pubmed-article:10465294 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:192274 | entrezgene:pubmed | pubmed-article:10465294 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10465294 | lld:pubmed |
