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pubmed-article:10464599pubmed:abstractTextTwo-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes, hMLH1.lld:pubmed
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pubmed-article:10464599pubmed:dateRevised2007-11-15lld:pubmed
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pubmed-article:10464599pubmed:articleTitleA simple system for automated two-dimensional electrophoresis: applications to genetic testing.lld:pubmed
pubmed-article:10464599pubmed:affiliationDepartment of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.lld:pubmed
pubmed-article:10464599pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10464599pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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