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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-10-12
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pubmed:abstractText |
Two-dimensional gene scanning (TDGS) is a method for mutation detection based on two-dimensional electrophoretic separation of PCR-amplified DNA fragments according to size and base pair sequence. To facilitate two-dimensional electrophoresis without manual interference, a simple three-chamber unit was designed on top of an existing vertical electrophoresis instrument. Application of this system to genetic testing is demonstrated by the performance of a complete two-dimensional mutational scanning assay of one of the hereditary nonpolyposis colon cancer syndrome susceptibility genes, hMLH1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1090-6576
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
67-70
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10464599-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10464599-Carrier Proteins,
pubmed-meshheading:10464599-Colorectal Neoplasms, Hereditary Nonpolyposis,
pubmed-meshheading:10464599-DNA Mutational Analysis,
pubmed-meshheading:10464599-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:10464599-Equipment Design,
pubmed-meshheading:10464599-Humans,
pubmed-meshheading:10464599-Neoplasm Proteins,
pubmed-meshheading:10464599-Nuclear Proteins,
pubmed-meshheading:10464599-Polymerase Chain Reaction
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pubmed:year |
1998
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pubmed:articleTitle |
A simple system for automated two-dimensional electrophoresis: applications to genetic testing.
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pubmed:affiliation |
Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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