Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-10-15
pubmed:abstractText
We developed an in vitro model to study the effect of anti-recoverin antibodies on retinal cells and the mechanism(s) by which they kill photoreceptors in cancer-associated retinopathy (CAR). Rat retinal cells were grown in a defined medium, and cell types were identified by using antibodies against rhodopsin, recoverin, syntaxin, and thy-1. Purified immunoglobulin (IgG) against recoverin was added to the cultures at different concentrations for 24, 48, or 72 hr, and the survival of the cells was determined by fluorescence microscopy. Preimmune IgG and normal medium were used as controls. The cell death detection enzyme-linked immunosorbent assay and the terminal deoxyuridine triphosphate nick-end labeling assay were used to demonstrate cells undergoing apoptosis. Double labeling was used to visualize cell types and apoptotic death. Rods, amacrine cells, and ganglion cells were identified in the cultures. Rod cells, but not ganglion cells and amacrine cells, markedly decreased in the presence of 200 microg/ml of anti-recoverin IgG for 24, 48, and 72 hr. Anti-recoverin antibodies caused apoptosis in rod cells but not in amacrine cells. Almost all cells were shown to take up IgG from the medium. In conclusion, our retinal cell cultures provide a system for investigating antibody-mediated photoreceptor cell death and demonstrate that anti-recoverin antibodies cause the apoptotic death of rod cells, with no effect on amacrine cells. The results suggest that anti-recoverin antibodies play a key role in the apoptotic death of photoreceptors in CAR.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hippocalcin, http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Qa-SNARE Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Rcvrn protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Recoverin, http://linkedlifedata.com/resource/pubmed/chemical/Rhodopsin
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
706-18
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10462694-Animals, pubmed-meshheading:10462694-Antibodies, pubmed-meshheading:10462694-Antigens, Neoplasm, pubmed-meshheading:10462694-Antigens, Thy-1, pubmed-meshheading:10462694-Apoptosis, pubmed-meshheading:10462694-Calcium-Binding Proteins, pubmed-meshheading:10462694-Cells, Cultured, pubmed-meshheading:10462694-Eye Proteins, pubmed-meshheading:10462694-Hippocalcin, pubmed-meshheading:10462694-In Situ Nick-End Labeling, pubmed-meshheading:10462694-Lipoproteins, pubmed-meshheading:10462694-Membrane Proteins, pubmed-meshheading:10462694-Nerve Tissue Proteins, pubmed-meshheading:10462694-Photoreceptor Cells, Vertebrate, pubmed-meshheading:10462694-Qa-SNARE Proteins, pubmed-meshheading:10462694-Rabbits, pubmed-meshheading:10462694-Rats, pubmed-meshheading:10462694-Recoverin, pubmed-meshheading:10462694-Retina, pubmed-meshheading:10462694-Retinal Ganglion Cells, pubmed-meshheading:10462694-Retinal Rod Photoreceptor Cells, pubmed-meshheading:10462694-Rhodopsin
pubmed:year
1999
pubmed:articleTitle
Anti-recoverin antibodies cause the apoptotic death of mammalian photoreceptor cells in vitro.
pubmed:affiliation
Department of Ophthalmology, Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA. weiheng_chen@ouhsc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't