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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0062503,
umls-concept:C0075691,
umls-concept:C0185117,
umls-concept:C0475264,
umls-concept:C0851285,
umls-concept:C1160340,
umls-concept:C1413128,
umls-concept:C1419895,
umls-concept:C1882598,
umls-concept:C2911684
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pubmed:issue |
17
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pubmed:dateCreated |
1999-9-23
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pubmed:abstractText |
The syndecan family of cell surface heparan sulfate proteoglycans interacts via their cytoplasmic C-terminal tail with the PDZ domain of CASK/LIN-2, a membrane-associated guanylate kinase homolog. The syndecan-CASK interaction may be involved in intercellular signaling and/or cell adhesion. Here we show that syndecan-1 to syndecan-4 have distinctive mRNA distributions in adult rat brain by in situ hybridization, with syndecan-2 and -3 being the major syndecans expressed in neurons of the forebrain. At the protein level, syndecan-2 and -3 are differentially localized within neurons; syndecan-3 is concentrated in axons, whereas syndecan-2 is localized in synapses. The synaptic accumulation of syndecan-2 occurs late in synapse development. CASK is a cytoplasmic-binding partner for syndecans, and its subcellular distribution changes strikingly during development, shifting from a primarily axonal distribution in the first 2 postnatal weeks to a somatodendritic distribution in adult brain. This change in CASK distribution correlates temporally and spatially with the expression patterns of syndecan-3 and -2, consistent with the association of both of these syndecans with CASK in vivo. In support of this, we were able to coimmunoprecipitate a complex of CASK and syndecan-3 from brain extracts. Our results indicate that specific syndecans are differentially expressed in various cell types of the brain and are targeted to distinct subcellular compartments in neurons, where they may serve specialized functions. Moreover, CASK is appropriately expressed and localized to interact with both syndecan-2 and -3 in different compartments of the neuron throughout postnatal development.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Heparan Sulfate Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sdc1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sdc2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sdc3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Sdc4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecan-1,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecan-2,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecan-3,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecan-4,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecans
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7415-25
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10460248-Aging,
pubmed-meshheading:10460248-Amino Acid Sequence,
pubmed-meshheading:10460248-Animals,
pubmed-meshheading:10460248-Brain,
pubmed-meshheading:10460248-COS Cells,
pubmed-meshheading:10460248-Embryonic and Fetal Development,
pubmed-meshheading:10460248-Gene Expression Regulation, Developmental,
pubmed-meshheading:10460248-Heparan Sulfate Proteoglycans,
pubmed-meshheading:10460248-Immunohistochemistry,
pubmed-meshheading:10460248-Membrane Glycoproteins,
pubmed-meshheading:10460248-Molecular Sequence Data,
pubmed-meshheading:10460248-Neurons,
pubmed-meshheading:10460248-Proteoglycans,
pubmed-meshheading:10460248-RNA, Messenger,
pubmed-meshheading:10460248-Rats,
pubmed-meshheading:10460248-Recombinant Proteins,
pubmed-meshheading:10460248-Sequence Alignment,
pubmed-meshheading:10460248-Sequence Homology, Amino Acid,
pubmed-meshheading:10460248-Subcellular Fractions,
pubmed-meshheading:10460248-Synapses,
pubmed-meshheading:10460248-Syndecan-1,
pubmed-meshheading:10460248-Syndecan-2,
pubmed-meshheading:10460248-Syndecan-3,
pubmed-meshheading:10460248-Syndecan-4,
pubmed-meshheading:10460248-Syndecans,
pubmed-meshheading:10460248-Transcription, Genetic,
pubmed-meshheading:10460248-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Regulated expression and subcellular localization of syndecan heparan sulfate proteoglycans and the syndecan-binding protein CASK/LIN-2 during rat brain development.
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pubmed:affiliation |
Howard Hughes Medical Institute and Department of Neurobiology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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