Source:http://linkedlifedata.com/resource/pubmed/id/10459638
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1999-9-7
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pubmed:abstractText |
Three molecular typing methods, pulsed-field gel electrophoresis (PFGE), ribotyping, and flagellin (flaA) gene typing, were used to discriminate within a group of 28 Campylobacter jejuni, heat-stable serotype 55 (HS55) isolates derived from cases of campylobacter enteritis occurring throughout Scotland, including 9 isolates associated with an outbreak. PFGE was found to be most discriminatory, identifying 6 distinct profiles, followed by ribotyping (5 profiles), and then flagellin gene typing (4 profiles). The coincidence of all three genotypic markers identified a dominant clonal line within the HS55 group, accounting for each of the outbreak strains, and for 9 of the 19 sporadic strains. A second, closely related, clonal line accounted for a further 5 of the sporadic strains, and also included the HS55 reference strain. Identification and monitoring of such clonal lines should facilitate more effective future epidemiological surveillance of C. jejuni.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0950-2688
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
367-75
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pubmed:dateRevised |
2010-3-3
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pubmed:meshHeading |
pubmed-meshheading:10459638-Campylobacter Infections,
pubmed-meshheading:10459638-Campylobacter jejuni,
pubmed-meshheading:10459638-DNA, Bacterial,
pubmed-meshheading:10459638-DNA Primers,
pubmed-meshheading:10459638-Disease Outbreaks,
pubmed-meshheading:10459638-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:10459638-Epidemiologic Methods,
pubmed-meshheading:10459638-Flagellin,
pubmed-meshheading:10459638-Genotype,
pubmed-meshheading:10459638-Humans,
pubmed-meshheading:10459638-Polymerase Chain Reaction,
pubmed-meshheading:10459638-Predictive Value of Tests,
pubmed-meshheading:10459638-RNA, Ribosomal, 16S,
pubmed-meshheading:10459638-Scotland,
pubmed-meshheading:10459638-Serotyping
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pubmed:year |
1999
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pubmed:articleTitle |
Molecular epidemiological investigation of an outbreak of Campylobacter jejuni identifies a dominant clonal line within Scottish serotype HS55 populations.
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pubmed:affiliation |
Department of Medical Microbiology, University of Aberdeen, Scotland, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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