Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-9-14
pubmed:abstractText
Mice deficient in lymphotoxin (LT)-alpha lack peripheral lymph nodes and Peyer's patches and have profound defects in development of follicular dendritic cell networks, germinal center formation, and T/B cell segregation in the spleen. Although LTalpha is known to be expressed by NK cells as well as T and B lymphocytes, the requirement of LTalpha for NK cell functions is largely unknown. To address this issue, we have assessed NK cell functions in LTalpha-deficient mice by evaluating tumor models with known requirements for NK cells to control their growth and metastasis. Syngeneic B16F10 melanoma cells inoculated s.c. grew more rapidly in LTalpha-/- mice than in the wild-type littermates, and the formation of experimental pulmonary metastases was significantly enhanced in LTalpha-/- mice. Although LTalpha-/- mice exhibited almost a normal total number of NK cells in spleen, they showed an impaired recruitment of NK cells to lung and liver. Additionally, lytic NK cells were not efficiently produced from LTalpha-/- bone marrow cells in vitro in the presence of IL-2 and IL-15. These data suggest that LTalpha signaling may be involved in the maturation and recruitment of NK cells and may play an important role in antitumor surveillance.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2809-15
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10453025-Animals, pubmed-meshheading:10453025-Bone Marrow Cells, pubmed-meshheading:10453025-Carcinoma, Lewis Lung, pubmed-meshheading:10453025-Cell Division, pubmed-meshheading:10453025-Cell Movement, pubmed-meshheading:10453025-Cytotoxicity, Immunologic, pubmed-meshheading:10453025-Hematopoietic Stem Cells, pubmed-meshheading:10453025-Immunologic Deficiency Syndromes, pubmed-meshheading:10453025-Killer Cells, Natural, pubmed-meshheading:10453025-Lung Neoplasms, pubmed-meshheading:10453025-Lymphocyte Activation, pubmed-meshheading:10453025-Lymphotoxin-alpha, pubmed-meshheading:10453025-Melanoma, Experimental, pubmed-meshheading:10453025-Mice, pubmed-meshheading:10453025-Mice, Inbred C57BL, pubmed-meshheading:10453025-Mice, Knockout, pubmed-meshheading:10453025-Mutagenesis, Site-Directed, pubmed-meshheading:10453025-Organ Specificity, pubmed-meshheading:10453025-Spleen
pubmed:year
1999
pubmed:articleTitle
Mice with a targeted mutation in lymphotoxin-alpha exhibit enhanced tumor growth and metastasis: impaired NK cell development and recruitment.
pubmed:affiliation
Laboratory of Molecular Immunoregulation, Division of Basic Sciences, Science Applications International Corp.-Frederick, National Cancer Institute-Frederic Cancer Research and Development Center, MD 21702, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't