Linked Life Data

10452994

Source:http://linkedlifedata.com/resource/pubmed/id/10452994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-9-14
pubmed:abstractText
CD8+ T cells from TCR transgenic 2C mice, specific for SIYRYYGL peptide bound to H-2Kb, were adoptively transferred into C57BL/6 recipients to allow monitoring of their location, numbers, and phenotype upon peptide challenge. Recipients were primed by s.c. injection of SIYRYYGL alone or with CFA or IL-12, and the transferred cells then tracked by flow cytometry using the 1B2 mAb specific for the 2C TCR. Peptide alone induced a transient and weak expansion of 1B2+ cells in the draining lymph nodes (DLN) by day 3, but these cells were tolerant to secondary peptide challenge. In contrast, priming with CFA/peptide resulted in a large clonal expansion of 1B2+ cells in DLN by day 3, and the cells exhibited a CD25highCD44high phenotype, blast transformation, and lytic effector function. By day 5, 1B2+ cell numbers decreased in the DLN and increased in the spleen and blood. 1B2+ cells with a memory phenotype persisted through day 60 in the DLN, spleen, and blood and responded to secondary peptide challenge. Immunization with peptide, along with IL-12, mimicked the adjuvant effects of CFA with respect to phenotype, clonal expansion, effector function, and establishment of memory. IL-12 was not unique in providing this adjuvant effect however, since CFA/peptide immunization of IL-12-deficient recipient mice also resulted in 1B2+ T cell activation and clonal expansion. Thus, CFA or IL-12 can enhance Ag-specific CD8+ T cell responses to peptide, demonstrating that an inflammatory cytokine(s) can support activation and prevent tolerance induction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2561-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10452994-Adjuvants, Immunologic, pubmed-meshheading:10452994-Adoptive Transfer, pubmed-meshheading:10452994-Animals, pubmed-meshheading:10452994-Antigens, pubmed-meshheading:10452994-CD8-Positive T-Lymphocytes, pubmed-meshheading:10452994-Clone Cells, pubmed-meshheading:10452994-Freund's Adjuvant, pubmed-meshheading:10452994-Immune Tolerance, pubmed-meshheading:10452994-Immunization, pubmed-meshheading:10452994-Immunologic Memory, pubmed-meshheading:10452994-Interleukin-12, pubmed-meshheading:10452994-Lymphocyte Activation, pubmed-meshheading:10452994-Mice, pubmed-meshheading:10452994-Mice, Inbred C57BL, pubmed-meshheading:10452994-Mice, Knockout, pubmed-meshheading:10452994-Mice, Transgenic, pubmed-meshheading:10452994-Oligopeptides, pubmed-meshheading:10452994-Solubility, pubmed-meshheading:10452994-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
Adjuvant effect of IL-12: conversion of peptide antigen administration from tolerizing to immunizing for CD8+ T cells in vivo.
pubmed:affiliation
Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis 55455, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.