Source:http://linkedlifedata.com/resource/pubmed/id/10449908
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
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| pubmed:dateCreated |
1999-10-15
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| pubmed:databankReference | |
| pubmed:abstractText |
The intron-containing genes encoding human and mouse ribosomal protein (r-protein) L27A were cloned and sequenced. The human r-protein L27A gene (RPL27A) shared an identical exon/intron structure with the mouse r-protein 27A gene (Rpl27a). The translational start codon ATG was separated from the main reading frame by the first intron sequence in both genes. An approximately 200-bp sequence upstream of the translational start site of both genes displayed remarkable similarity, and contained the putative promoters lacking canonical TATA, but harbored Sp1 binding sites and a short stretch of pyrimidine cluster, similar to other r-protein genes. Transcriptional regulatory elements, Box-A and GABP, found in the promoters of some other r-protein genes were also conserved in both genes. These structural features were included in the typical CpG island identified in the 5'-end sequences, suggesting that RPL27A/Rpl27a cloned here are authentic and transcriptionally active. Fluorescence in situ hybridization (FISH) analysis localized the mouse intron-containing Rpl27a to chromosome 7E2-F1 syntenic to human chromosome 11p15, where human RPL27A was located.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0301-0171
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
85
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
248-51
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10449908-Animals,
pubmed-meshheading:10449908-Base Sequence,
pubmed-meshheading:10449908-Blotting, Southern,
pubmed-meshheading:10449908-Chromosome Mapping,
pubmed-meshheading:10449908-Chromosomes, Human, Pair 11,
pubmed-meshheading:10449908-Cloning, Molecular,
pubmed-meshheading:10449908-Codon, Initiator,
pubmed-meshheading:10449908-CpG Islands,
pubmed-meshheading:10449908-Gene Library,
pubmed-meshheading:10449908-Humans,
pubmed-meshheading:10449908-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10449908-Mice,
pubmed-meshheading:10449908-Molecular Sequence Data,
pubmed-meshheading:10449908-Promoter Regions, Genetic,
pubmed-meshheading:10449908-Ribosomal Proteins,
pubmed-meshheading:10449908-Sequence Analysis, DNA,
pubmed-meshheading:10449908-Sequence Homology, Nucleic Acid
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| pubmed:year |
1999
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| pubmed:articleTitle |
Genomic structure and chromosome location of RPL27A/Rpl27a, the genes encoding human and mouse ribosomal protein L27A.
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| pubmed:affiliation |
Division of Genetic Resources, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. jkusuda@nih.go.jp
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| pubmed:publicationType |
Journal Article
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