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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1999-9-9
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pubmed:abstractText |
Basic helix-loop-helix (bHLH) DNA-binding proteins have been demonstrated to regulate tissue-specific transcription within multiple cell lineages. The Id family of helix-loop-helix proteins does not possess a basic DNA-binding domain and functions as a negative regulator of bHLH proteins. Overexpression of Id proteins within a variety of cell types has been shown to inhibit their ability to differentiate under appropriate conditions. We demonstrate that ectopic expression of Id-1 leads to activation of telomerase activity and immortalization of primary human keratinocytes. These immortalized cells have a decreased capacity to differentiate as well as activate phosphorylation of the retinoblastoma protein. Additionally, these cells acquire an impaired p53-mediated DNA-damage response as a late event in immortalization. We conclude that bHLH proteins play a pivotal role in regulating normal keratinocyte growth and differentiation, which can be disrupted by the immortalizing functions of Id-1 through activation of telomerase activity and inactivation of the retinoblastoma protein.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1052771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1372755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-14315085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1628620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1644289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1845902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1922066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-1944284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-2156629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-2204114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-2460337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-2476573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-7214336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-7637581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-7686954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-7760836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8269506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8288123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8294468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8598035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8598912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8649364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8658197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8692840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8710384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-8934878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9121427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9284049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9288757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9454332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9637678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10449746-9817205
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ID1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibitor of Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Telomerase,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/telomerase RNA
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9637-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10449746-Catalytic Domain,
pubmed-meshheading:10449746-Cell Aging,
pubmed-meshheading:10449746-Cells, Cultured,
pubmed-meshheading:10449746-DNA Damage,
pubmed-meshheading:10449746-DNA-Binding Proteins,
pubmed-meshheading:10449746-Enzyme Activation,
pubmed-meshheading:10449746-Helix-Loop-Helix Motifs,
pubmed-meshheading:10449746-Humans,
pubmed-meshheading:10449746-Inhibitor of Differentiation Protein 1,
pubmed-meshheading:10449746-Keratinocytes,
pubmed-meshheading:10449746-Phosphorylation,
pubmed-meshheading:10449746-RNA,
pubmed-meshheading:10449746-Repressor Proteins,
pubmed-meshheading:10449746-Retinoblastoma Protein,
pubmed-meshheading:10449746-Telomerase,
pubmed-meshheading:10449746-Transcription Factors,
pubmed-meshheading:10449746-Tumor Suppressor Protein p53
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pubmed:year |
1999
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pubmed:articleTitle |
Immortalization of primary human keratinocytes by the helix-loop-helix protein, Id-1.
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pubmed:affiliation |
Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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